Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 18697..19222 Replicon plasmid pCTXM15_005784
Accession NZ_CP028576
Organism Escherichia coli strain WCHEC005784

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag DBQ99_RS00155 Protein ID WP_001159868.1
Coordinates 18917..19222 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag DBQ99_RS00150 Protein ID WP_000813634.1
Coordinates 18697..18915 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBQ99_RS00135 15224..15921 + 698 Protein_19 IS1 family transposase -
DBQ99_RS00140 16058..16990 - 933 WP_000991832.1 hypothetical protein -
DBQ99_RS00145 16994..17989 - 996 WP_000246636.1 hypothetical protein -
DBQ99_RS00150 18697..18915 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DBQ99_RS00155 18917..19222 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
DBQ99_RS00160 19223..20029 + 807 WP_000016982.1 site-specific integrase -
DBQ99_RS00170 20806..21510 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
DBQ99_RS00175 21573..22067 - 495 Protein_26 IS4-like element ISVsa5 family transposase -
DBQ99_RS00185 22210..23070 + 861 WP_000557454.1 aminoglycoside N-acetyltransferase AAC(3)-IId -
DBQ99_RS26320 23083..23187 + 105 Protein_28 tunicamycin resistance protein -
DBQ99_RS00195 23239..23943 - 705 WP_001067858.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-15 / aac(3)-IId / blaTEM-1B / blaOXA-1 / aac(6')-Ib-cr / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) - 1..112422 112422
- inside IScluster/Tn aac(3)-IId / blaTEM-1B - 15418..25102 9684


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T101469 WP_001159868.1 NZ_CP028576:18917-19222 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T101469 NZ_CP028576:18917-19222 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT101469 WP_000813634.1 NZ_CP028576:18697-18915 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT101469 NZ_CP028576:18697-18915 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References