Detailed information of TA system TA101467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2474678..2475357
• Replicon: chromosome
• Accession: NZ_CP028574
• Organism: Acinetobacter pittii strain WCHAP005046

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S3TWT7
• Locus tag: DBQ26_RS13475
• Protein ID: WP_000838146.1
• Coordinates: 2475175..2475357 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: N9L2H6
• Locus tag: DBQ26_RS13470
• Protein ID: WP_000966688.1
• Coordinates: 2474678..2475082 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBQ26_RS13445	2470516..2471679	-	1164	WP_002045824.1	glutathione-dependent formaldehyde dehydrogenase	-
DBQ26_RS13450	2472121..2472537	+	417	WP_005070343.1	hypothetical protein	-
DBQ26_RS13455	2472544..2473503	-	960	Protein_2387	tape measure protein	-
DBQ26_RS13460	2473634..2473897	-	264	WP_001275792.1	hypothetical protein	-
DBQ26_RS13465	2473899..2474579	-	681	WP_000721572.1	hypothetical protein	-
DBQ26_RS13470	2474678..2475082	-	405	WP_000966688.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DBQ26_RS13475	2475175..2475357	-	183	WP_000838146.1	type II toxin-antitoxin system HicA family toxin	Toxin
DBQ26_RS13480	2475683..2476198	-	516	WP_025464105.1	hypothetical protein	-
DBQ26_RS13485	2476269..2477186	-	918	WP_002121543.1	hypothetical protein	-
DBQ26_RS13490	2477239..2478567	-	1329	WP_023187966.1	hypothetical protein	-
DBQ26_RS13495	2478567..2478920	-	354	WP_002121621.1	hypothetical protein	-
DBQ26_RS13500	2478989..2479387	-	399	WP_002121567.1	hypothetical protein	-
DBQ26_RS13505	2479389..2479757	-	369	WP_000539743.1	hypothetical protein	-
DBQ26_RS13510	2479729..2480133	-	405	WP_002121581.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2448711..2501818	53107
	-	flank	IS/Tn	-	-	2469632..2470378	746

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6695.84 Da
• Isoelectric Point: 10.5523

>T101467 WP_000838146.1 NZ_CP028574:c2475357-2475175 [Acinetobacter pittii]
VKSLDLIKMIEADGWYEVRVSGSHHHFKHPTKKGLVTIPHPKKDLPNGTVKSILKQAGLN

Nucleotide

• Length: 183 bp

>T101467 NZ_CP028574:c2475357-2475175 [Acinetobacter pittii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14647.69 Da
• Isoelectric Point: 4.4328

>AT101467 WP_000966688.1 NZ_CP028574:c2475082-2474678 [Acinetobacter pittii]
MLYPIAIERGSDTEAFGVTVPDIPGCFSAGDTLEEAIENVKEAISGHLEILAEDGEEIPLASELVKFVDDPEYKGMIWAV
TEVDVSRYLGKPEKINVTLPSRLIRKIDENVGKGKRYTTRSAFLAAGAEKLLHA

Nucleotide

• Length: 405 bp

>AT101467 NZ_CP028574:c2475082-2474678 [Acinetobacter pittii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Toxin

• AlphaFold DB: S3TWT7

Antitoxin

• AlphaFold DB: N9L2H6