Detailed information of TA system TA101453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 69914..70499
• Replicon: plasmid pNDM1_010045
• Accession: NZ_CP028560
• Organism: Acinetobacter sp. WCHA45

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N9M3U8
• Locus tag: CDG55_RS00550
• Protein ID: WP_005155676.1
• Coordinates: 70212..70499 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9LW73
• Locus tag: CDG55_RS00545
• Protein ID: WP_005155677.1
• Coordinates: 69914..70225 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG55_RS00505	65282..65629	+	348	Protein_69	IS4 family transposase	-
CDG55_RS00515	65708..66412	-	705	WP_001067790.1	IS6-like element IS1008 family transposase	-
CDG55_RS00520	66458..67467	+	1010	Protein_71	chloramphenicol/florfenicol efflux MFS transporter FloR	-
CDG55_RS00525	67495..67761	+	267	WP_077170324.1	LysR family transcriptional regulator	-
CDG55_RS00530	67797..68501	-	705	WP_005028420.1	IS6 family transposase	-
CDG55_RS00540	68744..69835	+	1092	Protein_74	IS4 family transposase	-
CDG55_RS00545	69914..70225	-	312	WP_005155677.1	BrnA antitoxin family protein	Antitoxin
CDG55_RS00550	70212..70499	-	288	WP_005155676.1	BrnT family toxin	Toxin
CDG55_RS00555	70802..71835	+	1034	Protein_77	ISNCY family transposase	-
CDG55_RS00560	71838..72032	-	195	Protein_78	IS4 family transposase	-
CDG55_RS00565	72036..72761	-	726	Protein_79	IS1 family transposase	-
CDG55_RS00570	72822..73058	-	237	Protein_80	IS4 family transposase	-
CDG55_RS00575	73185..74084	-	900	WP_005166156.1	ParB/RepB/Spo0J family partition protein	-
CDG55_RS00580	74103..74879	-	777	WP_005166158.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / blaNDM-1 / sul2 / floR / mph(E) / msr(E)	-	1..190170	190170
	-	inside	IScluster/Tn	blaNDM-1 / sul2 / floR	-	57499..73061	15562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11275.66 Da
• Isoelectric Point: 5.6113

>T101453 WP_005155676.1 NZ_CP028560:c70499-70212 [Acinetobacter sp. WCHA45]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T101453 NZ_CP028560:c70499-70212 [Acinetobacter sp. WCHA45]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTTGAAACTGCAAG
CCTTGTTTTTGAAGATCCTCTACGGATCTCAATCCAAGACAGACATACCAATGGGGAAGAGCGTTGGCAAACCATTGGTA
AGGTTAAAGGCGTACTGATGTTATTAGTCGCTCATACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACCAAAGCGGAGCGCAATCAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11984.58 Da
• Isoelectric Point: 6.7277

>AT101453 WP_005155677.1 NZ_CP028560:c70225-69914 [Acinetobacter sp. WCHA45]
MSMVRYTREELNENFSNKQDAEVEHLLAKGTVPDDQLDLSDIPEITDWSNAIRHGQFFRPVKQQTSVRLDADVLAWLKAQ
GKGYQTRMNKILREAMLKDLKNH

Nucleotide

• Length: 312 bp

>AT101453 NZ_CP028560:c70225-69914 [Acinetobacter sp. WCHA45]
ATGAGCATGGTTAGATATACACGCGAAGAGCTGAATGAAAATTTCAGCAACAAGCAAGATGCTGAAGTTGAACACTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGTAATGCTATTCGTC
ATGGGCAATTTTTTCGTCCAGTGAAGCAACAAACGTCAGTTCGTCTTGATGCGGATGTATTAGCATGGTTAAAAGCACAA
GGAAAAGGTTATCAAACACGTATGAATAAAATTCTGCGTGAAGCAATGCTAAAAGATTTAAAAAATCATTAA

Structures

Toxin

• AlphaFold DB: N9M3U8

Antitoxin

• AlphaFold DB: N9LW73