Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT-BrnA |
| Location | 60090..60678 | Replicon | plasmid pNDM1_010045 |
| Accession | NZ_CP028560 | ||
| Organism | Acinetobacter sp. WCHA45 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | N8SM64 |
| Locus tag | CDG55_RS00465 | Protein ID | WP_000438825.1 |
| Coordinates | 60391..60678 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | D3JXQ4 |
| Locus tag | CDG55_RS00460 | Protein ID | WP_004728120.1 |
| Coordinates | 60090..60404 (-) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CDG55_RS00430 | 55316..56347 | + | 1032 | WP_004201169.1 | protein-disulfide reductase DsbD N-terminal domain-containing protein | - |
| CDG55_RS00435 | 56358..56996 | - | 639 | WP_004201168.1 | phosphoribosylanthranilate isomerase | - |
| CDG55_RS00440 | 57001..57366 | - | 366 | WP_004201167.1 | bleomycin binding protein Ble-MBL | - |
| CDG55_RS00445 | 57370..58182 | - | 813 | WP_004201164.1 | subclass B1 metallo-beta-lactamase NDM-1 | - |
| CDG55_RS00450 | 58283..59308 | - | 1026 | WP_004994718.1 | IS30-like element ISAba125 family transposase | - |
| CDG55_RS00455 | 59471..60001 | + | 531 | WP_000172759.1 | hypothetical protein | - |
| CDG55_RS00460 | 60090..60404 | - | 315 | WP_004728120.1 | BrnA antitoxin family protein | Antitoxin |
| CDG55_RS00465 | 60391..60678 | - | 288 | WP_000438825.1 | BrnT family toxin | Toxin |
| CDG55_RS00470 | 60919..61689 | - | 771 | WP_032073134.1 | hypothetical protein | - |
| CDG55_RS00475 | 61748..62086 | + | 339 | WP_032073153.1 | hypothetical protein | - |
| CDG55_RS00480 | 62214..62918 | - | 705 | WP_001067784.1 | IS6-like element IS1006 family transposase | - |
| CDG55_RS00485 | 62982..63812 | + | 831 | Protein_66 | IS91-like element ISVsa3 family transposase | - |
| CDG55_RS00495 | 64048..64290 | - | 243 | Protein_67 | phosphoglucosamine mutase | - |
| CDG55_RS00500 | 64377..65192 | - | 816 | WP_001043260.1 | sulfonamide-resistant dihydropteroate synthase Sul2 | - |
| CDG55_RS00505 | 65282..65629 | + | 348 | Protein_69 | IS4 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(3)-IId / blaOXA-58 / blaNDM-1 / sul2 / floR / mph(E) / msr(E) | - | 1..190170 | 190170 | |
| - | inside | IScluster/Tn | blaNDM-1 / sul2 / floR | - | 57499..73061 | 15562 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11304.70 Da Isoelectric Point: 5.6205
>T101452 WP_000438825.1 NZ_CP028560:c60678-60391 [Acinetobacter sp. WCHA45]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
Download Length: 288 bp
>T101452 NZ_CP028560:c60678-60391 [Acinetobacter sp. WCHA45]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG
Antitoxin
Download Length: 105 a.a. Molecular weight: 12200.86 Da Isoelectric Point: 8.4628
>AT101452 WP_004728120.1 NZ_CP028560:c60404-60090 [Acinetobacter sp. WCHA45]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
Download Length: 315 bp
>AT101452 NZ_CP028560:c60404-60090 [Acinetobacter sp. WCHA45]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TVQ4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TRI9 |