Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 60090..60678 Replicon plasmid pNDM1_010045
Accession NZ_CP028560
Organism Acinetobacter sp. WCHA45

Toxin (Protein)


Gene name brnT Uniprot ID N8SM64
Locus tag CDG55_RS00465 Protein ID WP_000438825.1
Coordinates 60391..60678 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID D3JXQ4
Locus tag CDG55_RS00460 Protein ID WP_004728120.1
Coordinates 60090..60404 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDG55_RS00430 55316..56347 + 1032 WP_004201169.1 protein-disulfide reductase DsbD N-terminal domain-containing protein -
CDG55_RS00435 56358..56996 - 639 WP_004201168.1 phosphoribosylanthranilate isomerase -
CDG55_RS00440 57001..57366 - 366 WP_004201167.1 bleomycin binding protein Ble-MBL -
CDG55_RS00445 57370..58182 - 813 WP_004201164.1 subclass B1 metallo-beta-lactamase NDM-1 -
CDG55_RS00450 58283..59308 - 1026 WP_004994718.1 IS30-like element ISAba125 family transposase -
CDG55_RS00455 59471..60001 + 531 WP_000172759.1 hypothetical protein -
CDG55_RS00460 60090..60404 - 315 WP_004728120.1 BrnA antitoxin family protein Antitoxin
CDG55_RS00465 60391..60678 - 288 WP_000438825.1 BrnT family toxin Toxin
CDG55_RS00470 60919..61689 - 771 WP_032073134.1 hypothetical protein -
CDG55_RS00475 61748..62086 + 339 WP_032073153.1 hypothetical protein -
CDG55_RS00480 62214..62918 - 705 WP_001067784.1 IS6-like element IS1006 family transposase -
CDG55_RS00485 62982..63812 + 831 Protein_66 IS91-like element ISVsa3 family transposase -
CDG55_RS00495 64048..64290 - 243 Protein_67 phosphoglucosamine mutase -
CDG55_RS00500 64377..65192 - 816 WP_001043260.1 sulfonamide-resistant dihydropteroate synthase Sul2 -
CDG55_RS00505 65282..65629 + 348 Protein_69 IS4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId / blaOXA-58 / blaNDM-1 / sul2 / floR / mph(E) / msr(E) - 1..190170 190170
- inside IScluster/Tn blaNDM-1 / sul2 / floR - 57499..73061 15562


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11304.70 Da        Isoelectric Point: 5.6205

>T101452 WP_000438825.1 NZ_CP028560:c60678-60391 [Acinetobacter sp. WCHA45]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T101452 NZ_CP028560:c60678-60391 [Acinetobacter sp. WCHA45]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12200.86 Da        Isoelectric Point: 8.4628

>AT101452 WP_004728120.1 NZ_CP028560:c60404-60090 [Acinetobacter sp. WCHA45]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT101452 NZ_CP028560:c60404-60090 [Acinetobacter sp. WCHA45]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A8TVQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0A8TRI9

References