Detailed information of TA system TA101451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 48636..49220
• Replicon: plasmid pNDM1_010045
• Accession: NZ_CP028560
• Organism: Acinetobacter sp. WCHA45

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: CDG55_RS00390
• Protein ID: WP_000286964.1
• Coordinates: 48918..49220 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CDG55_RS00385
• Protein ID: WP_000985609.1
• Coordinates: 48636..48917 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG55_RS00360	45278..45724	-	447	Protein_42	AraC family ligand binding domain-containing protein	-
CDG55_RS00365	45852..46604	-	753	Protein_43	IS1-like element ISAba3 family transposase	-
CDG55_RS00370	46695..47537	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
CDG55_RS00375	47618..47986	+	369	Protein_45	IS1-like element ISAba3 family transposase	-
CDG55_RS00380	48137..48556	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
CDG55_RS00385	48636..48917	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
CDG55_RS00390	48918..49220	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDG55_RS00395	49271..50296	-	1026	WP_001988464.1	IS30-like element ISAba125 family transposase	-
CDG55_RS00400	50589..50873	+	285	WP_015056391.1	hypothetical protein	-
CDG55_RS00410	51084..52613	-	1530	WP_015056392.1	IS91 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / blaNDM-1 / sul2 / floR / mph(E) / msr(E)	-	1..190170	190170
	-	inside	IScluster/Tn	aac(3)-IId / blaOXA-58	-	36908..50296	13388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T101451 WP_000286964.1 NZ_CP028560:c49220-48918 [Acinetobacter sp. WCHA45]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T101451 NZ_CP028560:c49220-48918 [Acinetobacter sp. WCHA45]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT101451 WP_000985609.1 NZ_CP028560:c48917-48636 [Acinetobacter sp. WCHA45]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT101451 NZ_CP028560:c48917-48636 [Acinetobacter sp. WCHA45]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94