Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4211669..4212288 Replicon chromosome
Accession NZ_CP028555
Organism Klebsiella variicola strain WCHKP19

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CAY66_RS24755 Protein ID WP_002892050.1
Coordinates 4212070..4212288 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag CAY66_RS24750 Protein ID WP_008805436.1
Coordinates 4211669..4212043 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY66_RS24735 4206824..4208017 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CAY66_RS24740 4208040..4211186 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
CAY66_RS24750 4211669..4212043 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
CAY66_RS24755 4212070..4212288 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CAY66_RS24760 4212447..4213013 + 567 WP_087498985.1 maltose O-acetyltransferase -
CAY66_RS31395 4212985..4213113 - 129 Protein_4038 hypothetical protein -
CAY66_RS24765 4213150..4213620 + 471 WP_008805434.1 YlaC family protein -
CAY66_RS24770 4213589..4215046 - 1458 WP_087498984.1 PLP-dependent aminotransferase family protein -
CAY66_RS24775 4215147..4215845 + 699 WP_016160391.1 GNAT family N-acetyltransferase -
CAY66_RS24780 4215842..4215982 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CAY66_RS24785 4215982..4216245 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T101448 WP_002892050.1 NZ_CP028555:4212070-4212288 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T101448 NZ_CP028555:4212070-4212288 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAAATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT101448 WP_008805436.1 NZ_CP028555:4211669-4212043 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT101448 NZ_CP028555:4211669-4212043 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References