Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2703285..2704873 Replicon chromosome
Accession NZ_CP028555
Organism Klebsiella variicola strain WCHKP19

Toxin (Protein)


Gene name hipA Uniprot ID A0A1F2MCM0
Locus tag CAY66_RS17285 Protein ID WP_008804919.1
Coordinates 2703551..2704873 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A2V3KHJ7
Locus tag CAY66_RS17280 Protein ID WP_038422544.1
Coordinates 2703285..2703551 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY66_RS17260 2699097..2700251 - 1155 WP_008804924.1 FAD-dependent urate hydroxylase HpxO -
CAY66_RS17265 2700597..2701976 + 1380 WP_012968246.1 purine permease -
CAY66_RS17270 2701954..2702454 + 501 WP_008804922.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
CAY66_RS17275 2702451..2702777 + 327 WP_087498798.1 hydroxyisourate hydrolase -
CAY66_RS17280 2703285..2703551 + 267 WP_038422544.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
CAY66_RS17285 2703551..2704873 + 1323 WP_008804919.1 type II toxin-antitoxin system HipA family toxin Toxin
CAY66_RS17290 2704890..2706182 - 1293 WP_012968243.1 glycoside hydrolase family 10 protein -
CAY66_RS17295 2706315..2707319 - 1005 WP_087498799.1 type 1 fimbrial protein -
CAY66_RS17300 2707307..2709808 - 2502 WP_087498800.1 fimbrial biogenesis outer membrane usher protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 48911.26 Da        Isoelectric Point: 7.9109

>T101446 WP_008804919.1 NZ_CP028555:2703551-2704873 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T101446 NZ_CP028555:2703551-2704873 [Klebsiella variicola]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGCACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAATCTGCTGCCCGACAGCCCGCAGGTCCGCGATCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTGGGACGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCACCTCGAGGGGTTGCGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCCTACCAGT
CGGATATCCCTCTCGGGATGATTACCGGCCAGGATGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGGGCACTGGTGTATTCCGCAGGGCACCACGCCGACCACGCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCTAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAGTTTCTGTGCCTGGCGCTGGCCAGGGAACTGG
GCCTGGCGGTGCCGGAGGCAGAGATTATCACCACGCCGCGGATCCGCGCGCTGGCGGTGACCCGCTTTGACCGGCGCTGG
GCGCAGGAGGGGCGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCCTTTGGCATCCCGTCGTCGATGAAATA
TGAATCTGACGGCGGGCCGGGTATTGCGGCAATCATGGCCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCGCAG
ACTTTATGAAATTCATGGTTTTTCAGTGGCTGGTTGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGTGGCAGCTACCGTCTAACGCCGTTCTACGACATCATTTCGGCGTTTCCGGTGCTGGGAGGCACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACAGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCAACCGCCAAAGCGGTGAACTTTCCTGGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCACCCACGTCTGGCGGGCCATTACGGAAAATATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGTTATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9923.30 Da        Isoelectric Point: 8.5912

>AT101446 WP_038422544.1 NZ_CP028555:2703285-2703551 [Klebsiella variicola]
MTSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT101446 NZ_CP028555:2703285-2703551 [Klebsiella variicola]
ATGACTTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTATCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACACTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTAGGGGTGGTGATGACGCTGAACGCCAAAGCCACAGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2MCM0

Antitoxin

Source ID Structure
AlphaFold DB A0A2V3KHJ7

References