Detailed information of TA system TA101437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1769732..1770322
• Replicon: chromosome
• Accession: NZ_CP028555
• Organism: Klebsiella variicola strain WCHKP19

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: CAY66_RS12535
• Protein ID: WP_012541133.1
• Coordinates: 1769990..1770322 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A1F2LZQ3
• Locus tag: CAY66_RS12530
• Protein ID: WP_012541132.1
• Coordinates: 1769732..1769989 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY66_RS12510	1765202..1766476	+	1275	WP_087499212.1	hypothetical protein	-
CAY66_RS12515	1766522..1767490	-	969	WP_080930678.1	IS5-like element IS903B family transposase	-
CAY66_RS12520	1767567..1768829	+	1263	WP_087498507.1	DUF3320 domain-containing protein	-
CAY66_RS12525	1769192..1769452	+	261	WP_087498505.1	helix-turn-helix domain-containing protein	-
CAY66_RS12530	1769732..1769989	+	258	WP_012541132.1	antitoxin	Antitoxin
CAY66_RS12535	1769990..1770322	+	333	WP_012541133.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CAY66_RS12545	1770645..1772081	+	1437	WP_016161429.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
CAY66_RS12555	1772454..1773908	-	1455	WP_087498504.1	AMP nucleosidase	-
CAY66_RS12560	1774039..1774284	-	246	WP_008804171.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11819.67 Da
• Isoelectric Point: 10.1839

>T101437 WP_012541133.1 NZ_CP028555:1769990-1770322 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNVARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T101437 NZ_CP028555:1769990-1770322 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACGTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCCGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9420.91 Da
• Isoelectric Point: 4.4915

>AT101437 WP_012541132.1 NZ_CP028555:1769732-1769989 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT101437 NZ_CP028555:1769732-1769989 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1F2LZQ3