Detailed information of TA system TA101433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 317815..318401
• Replicon: chromosome
• Accession: NZ_CP028555
• Organism: Klebsiella variicola strain WCHKP19

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: CAY66_RS05390
• Protein ID: WP_012967118.1
• Coordinates: 318033..318401 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: CAY66_RS05385
• Protein ID: WP_004174006.1
• Coordinates: 317815..318036 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY66_RS05365	313971..314897	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
CAY66_RS05370	314894..316171	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
CAY66_RS05375	316168..316935	+	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
CAY66_RS05380	316937..317650	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
CAY66_RS05385	317815..318036	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CAY66_RS05390	318033..318401	+	369	WP_012967118.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CAY66_RS05395	318693..320009	+	1317	WP_008806974.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
CAY66_RS05400	320116..321003	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
CAY66_RS05405	321000..321845	+	846	WP_044643074.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
CAY66_RS05410	321847..322917	+	1071	WP_087498406.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	314894..323654	8760

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (26-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13584.98 Da
• Isoelectric Point: 8.6410

>T101433 WP_012967118.1 NZ_CP028555:318033-318401 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGMTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T101433 NZ_CP028555:318033-318401 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTATGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAACCCGGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT101433 WP_004174006.1 NZ_CP028555:317815-318036 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT101433 NZ_CP028555:317815-318036 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTACTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7