Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5880..6405 Replicon plasmid pCTXM15_020019
Accession NZ_CP028553
Organism Klebsiella variicola strain WCHKP19

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag CAY66_RS02540 Protein ID WP_013023785.1
Coordinates 5880..6185 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag CAY66_RS02545 Protein ID WP_001568025.1
Coordinates 6187..6405 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY66_RS02510 1567..2193 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
CAY66_RS02515 2313..2492 + 180 WP_006788217.1 hypothetical protein -
CAY66_RS02520 2956..3750 - 795 WP_004197635.1 site-specific integrase -
CAY66_RS02525 3948..4964 - 1017 WP_017899884.1 hypothetical protein -
CAY66_RS02530 4975..5289 - 315 WP_053389906.1 hypothetical protein -
CAY66_RS02535 5316..5711 - 396 WP_017899885.1 hypothetical protein -
CAY66_RS02540 5880..6185 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
CAY66_RS02545 6187..6405 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CAY66_RS02565 7223..7513 - 291 WP_013023783.1 hypothetical protein -
CAY66_RS02570 7510..8637 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
CAY66_RS02575 8671..10263 - 1593 WP_015344964.1 hypothetical protein -
CAY66_RS02580 10470..11249 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / blaTEM-1B / blaCTX-M-15 / qnrS1 - 1..117244 117244


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T101431 WP_013023785.1 NZ_CP028553:c6185-5880 [Klebsiella variicola]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T101431 NZ_CP028553:c6185-5880 [Klebsiella variicola]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT101431 WP_001568025.1 NZ_CP028553:c6405-6187 [Klebsiella variicola]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT101431 NZ_CP028553:c6405-6187 [Klebsiella variicola]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References