Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 38341..39138 Replicon plasmid p2_020019
Accession NZ_CP028550
Organism Klebsiella variicola strain WCHKP19

Toxin (Protein)


Gene name KacT Uniprot ID -
Locus tag CAY66_RS01650 Protein ID WP_187358053.1
Coordinates 38635..39138 (+) Length 168 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID -
Locus tag CAY66_RS01645 Protein ID WP_004109769.1
Coordinates 38341..38610 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY66_RS01625 34227..34952 - 726 WP_087499034.1 hypothetical protein -
CAY66_RS01630 35125..36843 - 1719 WP_087499033.1 hypothetical protein -
CAY66_RS01635 36886..37242 - 357 WP_162614700.1 hypothetical protein -
CAY66_RS01640 37248..37913 - 666 WP_087499032.1 AAA family ATPase -
CAY66_RS01645 38341..38610 + 270 WP_004109769.1 DUF1778 domain-containing protein Antitoxin
CAY66_RS01650 38635..39138 + 504 WP_187358053.1 GNAT family N-acetyltransferase Toxin
CAY66_RS31010 39166..39372 - 207 WP_023316023.1 hypothetical protein -
CAY66_RS31015 39323..39574 - 252 WP_004109798.1 hypothetical protein -
CAY66_RS01660 39576..40268 - 693 WP_087499029.1 hypothetical protein -
CAY66_RS01665 40282..40605 - 324 WP_004109805.1 hypothetical protein -
CAY66_RS01670 40696..42141 - 1446 WP_087499028.1 tail fiber domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..110186 110186


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(37-142)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 168 a.a.        Molecular weight: 18573.32 Da        Isoelectric Point: 9.2378

>T101430 WP_187358053.1 NZ_CP028550:38635-39138 [Klebsiella variicola]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANTNSLFYPTKSI
EELFEAV

Download         Length: 504 bp

>T101430 NZ_CP028550:38635-39138 [Klebsiella variicola]
ATGTTTTCAGCACAAGCCGTTTACGATTTTTCGGGCTTCGACTGCGGCGAACCATCTCTGAATGATTTCCTGCAAAACCG
CCTGGTACAGCAACACAACGGGCGTATATTACGTGGCTACCTGCTTCTTACCAAAGATCCGGTGCCGAAAGTTAAGGGTT
TTTACACGCTATCCGGGAGCTGTTTTGCAAGACAGACGCTACCATCGAACACCCAGAAGCGGAAAATCCCCTACGCAGAT
GCACCCAGCGTTACGCTTGGCCGTCTGGCAATAGATATCAGCCTACAGAAACAAGGCTACGGGGAGGTTCTCGTAACGCA
CGCGTTGAAGGTTGTTTATCAGGCTTCTCGCGCAGTAGGCATATACGCTTTGTTTGTGGATGCACTAAACCAAAGCGCAA
TGCAGTTCTATCAGAAGTTTGGATTCATCCCTTTGACGGGGGCGAACACCAACTCGCTTTTCTACCCCACGAAAAGCATT
GAAGAGCTGTTTGAAGCAGTGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10112.58 Da        Isoelectric Point: 7.0155

>AT101430 WP_004109769.1 NZ_CP028550:38341-38610 [Klebsiella variicola]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMNAISNPPAPNDRLKR
AAKRLQSME

Download         Length: 270 bp

>AT101430 NZ_CP028550:38341-38610 [Klebsiella variicola]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACGATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGCCGAT
TGATTCTTAGCGAAGAGTCCTGGAATCTGGTAATGAATGCTATCAGCAACCCGCCTGCACCGAATGATAGGCTGAAACGA
GCTGCCAAGCGTCTACAAAGCATGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References