Detailed information of TA system TA101429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 71680..72416
• Replicon: plasmid p1_020019
• Accession: NZ_CP028549
• Organism: Klebsiella variicola strain WCHKP19

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: CAY66_RS00405
• Protein ID: WP_003026803.1
• Coordinates: 71680..72162 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: CAY66_RS00410
• Protein ID: WP_003026799.1
• Coordinates: 72150..72416 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY66_RS00385	67009..68046	-	1038	WP_048293750.1	SIS domain-containing protein	-
CAY66_RS00390	68277..69374	+	1098	WP_048293751.1	LacI family transcriptional regulator	-
CAY66_RS00395	69878..70069	+	192	WP_048293752.1	hypothetical protein	-
CAY66_RS00400	70122..71468	-	1347	WP_077264177.1	ISNCY family transposase	-
CAY66_RS00405	71680..72162	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
CAY66_RS00410	72150..72416	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
CAY66_RS00420	72861..73091	-	231	WP_004206660.1	hypothetical protein	-
CAY66_RS00425	73105..73308	-	204	WP_004213596.1	hemolysin expression modulator Hha	-
CAY66_RS00430	73369..73860	-	492	WP_004206662.1	hypothetical protein	-
CAY66_RS00435	73994..74191	-	198	Protein_82	hypothetical protein	-
CAY66_RS00445	74244..74948	-	705	WP_032676094.1	IS6-like element IS26 family transposase	-
CAY66_RS00450	75012..76067	+	1056	Protein_84	transposase	-
CAY66_RS00455	76191..76667	+	477	WP_087499209.1	DNA starvation/stationary phase protection protein	-
CAY66_RS00460	76972..77322	-	351	WP_000928911.1	metalloregulator ArsR/SmtB family transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkF / mrkJ / pla	1..248112	248112
	-	inside	IScluster/Tn	-	-	70122..80192	10070

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T101429 WP_003026803.1 NZ_CP028549:c72162-71680 [Klebsiella variicola]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T101429 NZ_CP028549:c72162-71680 [Klebsiella variicola]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT101429 WP_003026799.1 NZ_CP028549:c72416-72150 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT101429 NZ_CP028549:c72416-72150 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66