101406

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 19182..19825 Replicon plasmid pKPC2_020143
Accession NZ_CP028547
Organism Klebsiella pneumoniae subsp. pneumoniae strain SCKP020143

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CLQ46_RS01825 Protein ID WP_001044770.1
Coordinates 19409..19825 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CLQ46_RS01820 Protein ID WP_001261282.1
Coordinates 19182..19412 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ46_RS01785 14405..15184 - 780 WP_013214009.1 site-specific integrase -
CLQ46_RS01790 15368..16372 - 1005 WP_011977814.1 hypothetical protein -
CLQ46_RS01795 16402..16605 - 204 WP_011977813.1 hypothetical protein -
CLQ46_RS01800 16651..17172 - 522 WP_013214008.1 hypothetical protein -
CLQ46_RS01805 17230..17580 - 351 WP_032738650.1 hypothetical protein -
CLQ46_RS01810 17659..18603 - 945 WP_011977810.1 hypothetical protein -
CLQ46_RS01815 18764..19198 - 435 WP_015493090.1 hypothetical protein -
CLQ46_RS01820 19182..19412 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CLQ46_RS01825 19409..19825 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CLQ46_RS01830 19899..21461 + 1563 WP_004206609.1 AAA family ATPase -
CLQ46_RS01835 21446..22468 + 1023 WP_000361404.1 helicase UvrD -
CLQ46_RS01840 22724..23421 + 698 Protein_35 IS1 family transposase -
CLQ46_RS01845 23747..24628 + 882 WP_004199234.1 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / rmtB / blaKPC-2 / blaSHV-12 / catA2 - 1..73791 73791


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T101406 WP_001044770.1 NZ_CP028547:19409-19825 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T101406 NZ_CP028547:19409-19825 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT101406 WP_001261282.1 NZ_CP028547:19182-19412 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT101406 NZ_CP028547:19182-19412 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References