Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 867424..868043 Replicon chromosome
Accession NZ_CP028542
Organism Klebsiella pneumoniae strain WCHKP2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CAY63_RS05875 Protein ID WP_002892050.1
Coordinates 867424..867642 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CAY63_RS05880 Protein ID WP_002892066.1
Coordinates 867669..868043 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY63_RS05845 863471..863734 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
CAY63_RS05850 863734..863874 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CAY63_RS05855 863871..864569 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
CAY63_RS05860 864670..866121 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
CAY63_RS05865 866096..866566 - 471 WP_002892026.1 YlaC family protein -
CAY63_RS05870 866699..867265 - 567 WP_002892030.1 maltose O-acetyltransferase -
CAY63_RS05875 867424..867642 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CAY63_RS05880 867669..868043 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CAY63_RS05885 868529..871675 - 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
CAY63_RS05890 871698..872891 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T101388 WP_002892050.1 NZ_CP028542:c867642-867424 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T101388 NZ_CP028542:c867642-867424 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT101388 WP_002892066.1 NZ_CP028542:c868043-867669 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT101388 NZ_CP028542:c868043-867669 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References