Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 99390..100033 Replicon plasmid pKPC2_020002
Accession NZ_CP028541
Organism Klebsiella pneumoniae strain WCHKP2

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CAY63_RS00820 Protein ID WP_001044770.1
Coordinates 99390..99806 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CAY63_RS00825 Protein ID WP_001261282.1
Coordinates 99803..100033 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY63_RS00800 95493..95765 - 273 Protein_135 IS5/IS1182 family transposase -
CAY63_RS00805 95794..96491 - 698 Protein_136 IS1 family transposase -
CAY63_RS00810 96747..97769 - 1023 WP_000361404.1 helicase UvrD -
CAY63_RS00815 97754..99316 - 1563 WP_004206609.1 AAA family ATPase -
CAY63_RS00820 99390..99806 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CAY63_RS00825 99803..100033 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CAY63_RS00830 100017..100451 + 435 WP_015493090.1 hypothetical protein -
CAY63_RS00835 100612..101556 + 945 WP_011977810.1 hypothetical protein -
CAY63_RS00840 101635..101985 + 351 WP_032738650.1 hypothetical protein -
CAY63_RS00845 102043..102564 + 522 WP_013214008.1 hypothetical protein -
CAY63_RS00850 102610..102813 + 204 WP_011977813.1 hypothetical protein -
CAY63_RS00855 102843..103847 + 1005 WP_011977814.1 hypothetical protein -
CAY63_RS00860 104031..104810 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2 - 1..177516 177516


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T101381 WP_001044770.1 NZ_CP028541:c99806-99390 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T101381 NZ_CP028541:c99806-99390 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT101381 WP_001261282.1 NZ_CP028541:c100033-99803 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT101381 NZ_CP028541:c100033-99803 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References