Detailed information of TA system TA101380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 7079..7659
• Replicon: plasmid p1_020002
• Accession: NZ_CP028539
• Organism: Klebsiella pneumoniae strain WCHKP2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: CAY63_RS00050
• Protein ID: WP_071177730.1
• Coordinates: 7345..7659 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: CAY63_RS00045
• Protein ID: WP_000093040.1
• Coordinates: 7079..7357 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY63_RS00015	3139..3288	-	150	WP_032440454.1	colicin release lysis protein	-
CAY63_RS00020	3373..3630	-	258	WP_032440455.1	cloacin	-
CAY63_RS00025	3640..5325	-	1686	WP_032440457.1	cloacin	-
CAY63_RS00030	5652..5897	+	246	WP_032440458.1	hypothetical protein	-
CAY63_RS00035	6171..6542	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
CAY63_RS00040	6539..6745	+	207	Protein_7	TonB family protein	-
CAY63_RS00045	7079..7357	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
CAY63_RS00050	7345..7659	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CAY63_RS00055	7823..8251	+	429	WP_001140599.1	hypothetical protein	-
CAY63_RS00060	8277..8456	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
CAY63_RS00065	8483..9013	-	531	WP_071177729.1	hypothetical protein	-
CAY63_RS00070	9020..9751	-	732	WP_071177728.1	replication-relaxation family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T101380 WP_071177730.1 NZ_CP028539:7345-7659 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T101380 NZ_CP028539:7345-7659 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT101380 WP_000093040.1 NZ_CP028539:7079-7357 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT101380 NZ_CP028539:7079-7357 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1