Detailed information of TA system TA101377

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3569614..3570281
• Replicon: chromosome
• Accession: NZ_CP028538
• Organism: Enterobacter hormaechei strain SCEH020042

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: DBP88_RS19435
• Protein ID: WP_057064441.1
• Coordinates: 3569952..3570281 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: DBP88_RS19430
• Protein ID: WP_007870247.1
• Coordinates: 3569614..3569931 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBP88_RS19410	3565346..3567541	+	2196	WP_000093087.1	DEAD/DEAH box helicase	-
DBP88_RS19415	3568395..3568874	+	480	WP_108168719.1	antirestriction protein	-
DBP88_RS19420	3568890..3569366	+	477	WP_057064440.1	RadC family protein	-
DBP88_RS19425	3569375..3569596	+	222	WP_001548165.1	DUF987 domain-containing protein	-
DBP88_RS19430	3569614..3569931	+	318	WP_007870247.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DBP88_RS19435	3569952..3570281	+	330	WP_057064441.1	TA system toxin CbtA family protein	Toxin
DBP88_RS19440	3570966..3571892	-	927	WP_023306741.1	helix-turn-helix domain-containing protein	-
DBP88_RS19445	3572042..3573766	-	1725	WP_023306742.1	flagellin	-
DBP88_RS25640	3574536..3574958	-	423	WP_032671399.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3568395..3610018	41623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12424.59 Da
• Isoelectric Point: 10.1006

>T101377 WP_057064441.1 NZ_CP028538:3569952-3570281 [Enterobacter hormaechei]
MKTLPATISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFWDQTVIKEHIDAGITLADAVNFLVDKYELVRIDRKGF
SWQEQTPYLSVVDILRARRSTGLLKAKVK

Nucleotide

• Length: 330 bp

>T101377 NZ_CP028538:3569952-3570281 [Enterobacter hormaechei]
ATGAAAACTCTACCTGCTACAATTTCTCGGGCGGCGAAGCCTTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTTCTTAC
ACGGCTACTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGGGATCAAACTGTTATTAAGGAACATATTG
ATGCAGGGATCACCCTGGCCGATGCAGTCAACTTTCTGGTGGATAAGTACGAACTAGTTCGTATCGACCGGAAGGGATTT
TCGTGGCAAGAGCAGACCCCGTATCTTTCCGTAGTGGATATTCTGCGAGCAAGGCGCTCTACCGGATTGCTAAAAGCCAA
GGTGAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11839.44 Da
• Isoelectric Point: 6.9459

>AT101377 WP_007870247.1 NZ_CP028538:3569614-3569931 [Enterobacter hormaechei]
MNNTTWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECLKLDVAFPHFISQMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT101377 NZ_CP028538:3569614-3569931 [Enterobacter hormaechei]
ATGAACAACACCACATGGGGTCTGCAGCGGGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAATGCCTGAAGTTGGATGTCGCATTCCCGC
ATTTTATCAGTCAGATGGAATCAATGCTGACTACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACTCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATATATCGCCATTTATCCCACTCAACGCTAA