Detailed information of TA system TA101368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2309687..2310426
• Replicon: chromosome
• Accession: NZ_CP028538
• Organism: Enterobacter hormaechei strain SCEH020042

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: DBP88_RS13280
• Protein ID: WP_032624244.1
• Coordinates: 2309687..2310172 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: DBP88_RS13285
• Protein ID: WP_003857131.1
• Coordinates: 2310160..2310426 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBP88_RS13250	2305248..2305832	-	585	WP_023306207.1	NUDIX hydrolase	-
DBP88_RS13255	2305919..2306677	+	759	WP_023306208.1	DeoR/GlpR family DNA-binding transcription regulator	-
DBP88_RS13260	2306782..2308074	+	1293	WP_023306209.1	hypothetical protein	-
DBP88_RS13265	2308374..2308655	+	282	Protein_2128	DUF4225 domain-containing protein	-
DBP88_RS13270	2308630..2308959	-	330	WP_023306210.1	hypothetical protein	-
DBP88_RS13275	2309092..2309661	+	570	WP_023306211.1	hypothetical protein	-
DBP88_RS13280	2309687..2310172	-	486	WP_032624244.1	GNAT family N-acetyltransferase	Toxin
DBP88_RS13285	2310160..2310426	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
DBP88_RS13290	2310490..2311419	-	930	WP_023306213.1	LysR family transcriptional regulator	-
DBP88_RS13295	2311549..2312937	+	1389	WP_023306214.1	MFS transporter	-
DBP88_RS13300	2312916..2313473	-	558	WP_023300131.1	OmpH family outer membrane protein	-
DBP88_RS13305	2313594..2314730	-	1137	WP_032676232.1	type 1 fimbrial protein	-
DBP88_RS13310	2314754..2315278	-	525	WP_087878309.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2294670..2310426	15756

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17531.27 Da
• Isoelectric Point: 10.1788

>T101368 WP_032624244.1 NZ_CP028538:c2310172-2309687 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEKAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T101368 NZ_CP028538:c2310172-2309687 [Enterobacter hormaechei]
GTGGGACGTGTAACCGCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTACTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AAAAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT101368 WP_003857131.1 NZ_CP028538:c2310426-2310160 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT101368 NZ_CP028538:c2310426-2310160 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTACA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9