Detailed information of TA system TA101365

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 98030..98712
• Replicon: chromosome
• Accession: NZ_CP028538
• Organism: Enterobacter hormaechei strain SCEH020042

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: DBP88_RS02670
• Protein ID: WP_108168483.1
• Coordinates: 98030..98371 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: DBP88_RS02675
• Protein ID: WP_108168484.1
• Coordinates: 98392..98712 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBP88_RS02635	93534..94013	+	480	WP_022646606.1	hypothetical protein	-
DBP88_RS02640	94256..95137	+	882	WP_032670896.1	helix-turn-helix domain-containing protein	-
DBP88_RS02645	95202..95501	-	300	WP_023299004.1	XRE family transcriptional regulator	-
DBP88_RS02650	95625..95966	-	342	WP_023305175.1	SymE family type I addiction module toxin	-
DBP88_RS02655	96094..96255	+	162	WP_065212817.1	DUF3950 domain-containing protein	-
DBP88_RS02660	96368..96574	-	207	WP_071788007.1	hypothetical protein	-
DBP88_RS02665	97083..97916	-	834	WP_099530882.1	DUF4942 domain-containing protein	-
DBP88_RS02670	98030..98371	-	342	WP_108168483.1	TA system toxin CbtA family protein	Toxin
DBP88_RS02675	98392..98712	-	321	WP_108168484.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DBP88_RS02680	98731..98952	-	222	WP_108168485.1	DUF987 domain-containing protein	-
DBP88_RS02685	98962..99438	-	477	WP_108168486.1	RadC family protein	-
DBP88_RS02690	99454..99912	-	459	WP_108168487.1	antirestriction protein	-
DBP88_RS02695	99993..100229	-	237	WP_108168488.1	DUF905 domain-containing protein	-
DBP88_RS02700	100307..100717	-	411	WP_043082399.1	hypothetical protein	-
DBP88_RS02705	100803..101417	-	615	WP_108168489.1	hypothetical protein	-
DBP88_RS02710	101414..102118	-	705	WP_108168490.1	WYL domain-containing protein	-
DBP88_RS02715	102319..103152	-	834	WP_108168491.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	92744..148943	56199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-105)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12956.85 Da
• Isoelectric Point: 7.2015

>T101365 WP_108168483.1 NZ_CP028538:c98371-98030 [Enterobacter hormaechei]
MKPQPATTSRAAELYLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHIDAGIMLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNAVR

Nucleotide

• Length: 342 bp

>T101365 NZ_CP028538:c98371-98030 [Enterobacter hormaechei]
ATGAAACCTCAACCTGCAACAACTTCGCGGGCGGCTGAGCTATATCTGTCGCCAGTGGCGGTGTGGCAAATGCTACTGAC
ACGCCTGCTGGAGCAGCACTATGGCCTGACACTGAATGACACGCCATTTAGTGATGAAACCGTTATTCAGGAACACATTG
ATGCCGGGATCATGCTGGCCGATGCTGTGAATTTTCTGGTGGAGAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCGCCTTATCTTCGGGCTGTAGATATTTTACGGGCAAGACAGGCAACTGGCCTGTTGCGACAAAG
CCGTAACAACGCAGTACGGTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12027.71 Da
• Isoelectric Point: 6.9370

>AT101365 WP_108168484.1 NZ_CP028538:c98712-98392 [Enterobacter hormaechei]
MRSNITWGLQRDITPCMGARLVQEGNRLHYLADRASITGKFSDDECLKLDVVFPHFISRIESMLTTGELNPRHAHCITIY
HNGFTCEADTLGSCAYVYVVVYSTQR

Nucleotide

• Length: 321 bp

>AT101365 NZ_CP028538:c98712-98392 [Enterobacter hormaechei]
ATGAGAAGTAACATCACATGGGGCCTGCAGCGGGATATCACGCCGTGCATGGGAGCACGTCTGGTGCAGGAGGGTAACCG
GCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGACGAATGTCTTAAGCTGGATGTGGTATTTC
CACATTTTATCAGCCGGATCGAGTCTATGCTGACCACTGGTGAATTGAATCCCCGTCATGCCCACTGCATCACCATTTAC
CACAACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGCCTACGTATATGTAGTCGTCTATTCCACTCAACGCTA
A