Detailed information of TA system TA101361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4425802..4426422
• Replicon: chromosome
• Accession: NZ_CP028520
• Organism: Lelliottia sp. WB101

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A2S5C408
• Locus tag: DAI21_RS21440
• Protein ID: WP_012016344.1
• Coordinates: 4426204..4426422 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: DAI21_RS21435
• Protein ID: WP_100777374.1
• Coordinates: 4425802..4426176 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DAI21_RS21425	4420928..4422121	+	1194	WP_100777372.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
DAI21_RS21430	4422144..4425290	+	3147	WP_100777373.1	multidrug efflux RND transporter permease subunit AcrB	-
DAI21_RS21435	4425802..4426176	+	375	WP_100777374.1	Hha toxicity modulator TomB	Antitoxin
DAI21_RS21440	4426204..4426422	+	219	WP_012016344.1	hemolysin expression modulator Hha	Toxin
DAI21_RS21445	4426638..4427189	+	552	WP_107704127.1	maltose O-acetyltransferase	-
DAI21_RS21450	4427307..4427774	+	468	WP_100777376.1	hypothetical protein	-
DAI21_RS21455	4427746..4429203	-	1458	WP_107704128.1	PLP-dependent aminotransferase family protein	-
DAI21_RS21460	4429304..4430014	+	711	WP_107704129.1	GNAT family N-acetyltransferase	-
DAI21_RS21465	4430011..4430151	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
DAI21_RS21470	4430154..4430414	-	261	WP_095281030.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8631.05 Da
• Isoelectric Point: 8.9039

>T101361 WP_012016344.1 NZ_CP028520:4426204-4426422 [Lelliottia sp. WB101]
MSDKPLTKTDYLMRLRRCQSLDTLERVIEKNKYELPDHELAVFYSAADHRLAELTMNKLYDKIPVSVWKFVR

Nucleotide

• Length: 219 bp

>T101361 NZ_CP028520:4426204-4426422 [Lelliottia sp. WB101]
ATGTCTGATAAACCATTAACAAAGACAGATTATTTGATGCGCTTGCGACGCTGCCAGTCACTGGACACCCTGGAGCGTGT
TATTGAAAAGAATAAATATGAACTTCCCGATCATGAACTGGCTGTATTTTATTCAGCAGCCGACCACCGCCTTGCAGAGT
TAACGATGAATAAACTGTATGACAAAATCCCAGTATCTGTGTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14521.26 Da
• Isoelectric Point: 5.1481

>AT101361 WP_100777374.1 NZ_CP028520:4425802-4426176 [Lelliottia sp. WB101]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFINASRANPVSHSC

Nucleotide

• Length: 375 bp

>AT101361 NZ_CP028520:4425802-4426176 [Lelliottia sp. WB101]
ATGGACGAATACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTTAATGATCCGACCTCCGCAATCAATTTGCAGCTAAATGAACTCATAGAGC
ATATAGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAATTGATTGAGCAAATTGATGAATATCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACACACAAGATTTGCAAAAATGGCGTAAATCGGGAAACCGCCT
ATTCCGTTGTTTCATCAACGCGAGCAGAGCTAACCCGGTCAGTCATTCATGTTAA

Structures

Toxin

• AlphaFold DB: A0A2S5C408