Detailed information of TA system TA101350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2395260..2396238
• Replicon: chromosome
• Accession: NZ_CP028516
• Organism: Bacillus cereus strain 1000305

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: FORC085_RS12150
• Protein ID: WP_000624977.1
• Coordinates: 2395260..2395997 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: FORC085_RS12155
• Protein ID: WP_000237818.1
• Coordinates: 2396110..2396238 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC085_RS12130	2390902..2391684	+	783	WP_000381792.1	class I SAM-dependent methyltransferase	-
FORC085_RS12135	2391842..2393527	-	1686	WP_033695152.1	alpha-keto acid decarboxylase family protein	-
FORC085_RS12140	2393635..2394117	+	483	WP_000191886.1	MarR family winged helix-turn-helix transcriptional regulator	-
FORC085_RS12145	2394284..2395021	+	738	WP_000594136.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
FORC085_RS12150	2395260..2395997	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
FORC085_RS12155	2396110..2396238	+	129	WP_000237818.1	hypothetical protein	Antitoxin
FORC085_RS12160	2396311..2396487	+	177	WP_000852629.1	stage II sporulation protein SB	-
FORC085_RS12165	2396506..2396895	-	390	WP_025709616.1	YxeA family protein	-
FORC085_RS12170	2397107..2398576	+	1470	WP_000287543.1	beta-Ala-His dipeptidase	-
FORC085_RS12175	2398696..2400038	+	1343	WP_088859367.1	IS3 family transposase	-
FORC085_RS12180	2400293..2401102	+	810	WP_000664543.1	C39 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T101350 WP_000624977.1 NZ_CP028516:2395260-2395997 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T101350 NZ_CP028516:2395260-2395997 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCAAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCAAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCTGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCACAATTTAGTATTATCGTCCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTGGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT101350 WP_000237818.1 NZ_CP028516:2396110-2396238 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT101350 NZ_CP028516:2396110-2396238 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAGGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5