Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | PfiT-PfiA/ParE(toxin) |
| Location | 5000401..5001012 | Replicon | chromosome |
| Accession | NZ_CP028490 | ||
| Organism | Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group) | ||
Toxin (Protein)
| Gene name | PfiT | Uniprot ID | Q4ZME8 |
| Locus tag | DA456_RS22280 | Protein ID | WP_003304103.1 |
| Coordinates | 5000662..5001012 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | PfiA | Uniprot ID | Q4ZME7 |
| Locus tag | DA456_RS22275 | Protein ID | WP_002556076.1 |
| Coordinates | 5000401..5000649 (+) | Length | 83 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DA456_RS22240 | 4995697..4995972 | + | 276 | WP_025168237.1 | hypothetical protein | - |
| DA456_RS22245 | 4996118..4996339 | + | 222 | Protein_4225 | hypothetical protein | - |
| DA456_RS22250 | 4996533..4997480 | - | 948 | WP_003173940.1 | aromatic alcohol reductase | - |
| DA456_RS22255 | 4997577..4998035 | + | 459 | WP_003429427.1 | helix-turn-helix transcriptional regulator | - |
| DA456_RS22260 | 4998228..4998443 | + | 216 | WP_003316224.1 | hypothetical protein | - |
| DA456_RS22265 | 4998716..4998976 | + | 261 | WP_003316225.1 | hypothetical protein | - |
| DA456_RS22270 | 4999102..5000064 | - | 963 | WP_004415744.1 | tyrosine-type recombinase/integrase | - |
| DA456_RS22275 | 5000401..5000649 | + | 249 | WP_002556076.1 | type II toxin-antitoxin system prevent-host-death family antitoxin | Antitoxin |
| DA456_RS22280 | 5000662..5001012 | + | 351 | WP_003304103.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| DA456_RS22285 | 5001375..5001596 | - | 222 | WP_003422691.1 | hypothetical protein | - |
| DA456_RS22290 | 5001664..5003049 | - | 1386 | WP_003422693.1 | P-type conjugative transfer protein TrbL | - |
| DA456_RS22295 | 5003253..5004008 | - | 756 | WP_029571664.1 | P-type conjugative transfer protein TrbJ | - |
| DA456_RS22300 | 5004051..5004425 | - | 375 | WP_003316229.1 | conjugal transfer transcriptional regulator TraJ | - |
| DA456_RS22305 | 5004704..5004994 | - | 291 | WP_003422698.1 | hypothetical protein | - |
| DA456_RS22310 | 5005061..5005786 | - | 726 | WP_050492103.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4993677..5007501 | 13824 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12973.76 Da Isoelectric Point: 4.7690
>T101347 WP_003304103.1 NZ_CP028490:5000662-5001012 [Pseudomonas syringae pv. atrofaciens]
MNTFALRFTDVAQQSLEDQVEHLAVHQGFSSAAQRIDTLIDAIQDKLLSTPLGYPVSHQLSELGVLHYRELNTDGYRIFY
EVRDAGDINVIVVALVLGSKQSVEQALIRYCLLQAI
MNTFALRFTDVAQQSLEDQVEHLAVHQGFSSAAQRIDTLIDAIQDKLLSTPLGYPVSHQLSELGVLHYRELNTDGYRIFY
EVRDAGDINVIVVALVLGSKQSVEQALIRYCLLQAI
Download Length: 351 bp
>T101347 NZ_CP028490:5000662-5001012 [Pseudomonas syringae pv. atrofaciens]
ATGAATACGTTTGCACTGCGCTTTACCGATGTTGCCCAGCAAAGCCTTGAGGATCAGGTTGAACACCTGGCTGTCCATCA
GGGATTCTCATCAGCTGCTCAACGCATAGATACCCTTATCGATGCCATCCAAGACAAGCTCCTGTCTACCCCGTTGGGTT
ACCCGGTCAGTCATCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGCTGAACACTGACGGATACCGGATCTTTTAC
GAGGTGAGGGACGCAGGCGATATAAATGTCATCGTCGTGGCGCTCGTGTTAGGTAGCAAGCAAAGTGTTGAACAGGCCCT
CATCCGCTACTGCCTGCTTCAGGCTATATAG
ATGAATACGTTTGCACTGCGCTTTACCGATGTTGCCCAGCAAAGCCTTGAGGATCAGGTTGAACACCTGGCTGTCCATCA
GGGATTCTCATCAGCTGCTCAACGCATAGATACCCTTATCGATGCCATCCAAGACAAGCTCCTGTCTACCCCGTTGGGTT
ACCCGGTCAGTCATCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGCTGAACACTGACGGATACCGGATCTTTTAC
GAGGTGAGGGACGCAGGCGATATAAATGTCATCGTCGTGGCGCTCGTGTTAGGTAGCAAGCAAAGTGTTGAACAGGCCCT
CATCCGCTACTGCCTGCTTCAGGCTATATAG
Antitoxin
Download Length: 83 a.a. Molecular weight: 9366.73 Da Isoelectric Point: 9.9579
>AT101347 WP_002556076.1 NZ_CP028490:5000401-5000649 [Pseudomonas syringae pv. atrofaciens]
MRVETISFLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFT
QK
MRVETISFLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFT
QK
Download Length: 249 bp
>AT101347 NZ_CP028490:5000401-5000649 [Pseudomonas syringae pv. atrofaciens]
ATGCGAGTTGAAACAATCAGTTTTCTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCATTGGTCGTCACGCAAAA
TGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCTGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CAATCAGCTCCCGCGAGTACTCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTACC
CAGAAATAA
ATGCGAGTTGAAACAATCAGTTTTCTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCATTGGTCGTCACGCAAAA
TGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCTGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CAATCAGCTCCCGCGAGTACTCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTACC
CAGAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0P9GDS9 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0P9GM89 |