Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3298571..3299205 Replicon chromosome
Accession NZ_CP028490
Organism Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)

Toxin (Protein)


Gene name vapC Uniprot ID A0A0P9KYQ9
Locus tag DA456_RS14285 Protein ID WP_024684220.1
Coordinates 3298801..3299205 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0P9J133
Locus tag DA456_RS14280 Protein ID WP_003421652.1
Coordinates 3298571..3298801 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DA456_RS14255 3293645..3294670 + 1026 WP_017277971.1 TRAP transporter substrate-binding protein -
DA456_RS14260 3294734..3295261 + 528 WP_003408544.1 TRAP transporter small permease -
DA456_RS14265 3295262..3296542 + 1281 WP_003421655.1 TRAP transporter large permease -
DA456_RS14270 3296627..3297598 + 972 WP_024649225.1 DMT family transporter -
DA456_RS14275 3297573..3298454 + 882 WP_029571921.1 aldose 1-epimerase -
DA456_RS14280 3298571..3298801 + 231 WP_003421652.1 antitoxin Antitoxin
DA456_RS14285 3298801..3299205 + 405 WP_024684220.1 tRNA(fMet)-specific endonuclease VapC Toxin
DA456_RS14290 3299430..3299942 + 513 WP_025168157.1 sigma-70 family RNA polymerase sigma factor -
DA456_RS14295 3299942..3300604 + 663 WP_003421647.1 ChrR family anti-sigma-E factor -
DA456_RS14300 3300627..3301883 - 1257 WP_004419198.1 3-phosphoshikimate 1-carboxyvinyltransferase -
DA456_RS14305 3301980..3302462 - 483 WP_029572576.1 PAS domain-containing protein -
DA456_RS14310 3302466..3303470 - 1005 WP_003421645.1 response regulator -
DA456_RS14315 3303736..3304185 + 450 WP_003345986.1 DUF2384 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

SpoVT-AbrB domain

(7-34)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14976.24 Da        Isoelectric Point: 6.8620

>T101346 WP_024684220.1 NZ_CP028490:3298801-3299205 [Pseudomonas syringae pv. atrofaciens]
MLKYMLDTNICIFTIKNKPTSVREAFNLHHGQLCISAITLMELVYGAEKSLSPERNLAVVEGFTARLEVLPYDSDAAAHT
GMIRAELARSSTPIGPYDQMIAGHARSLGLVVITNNQREFRRVEGLRVEDWVSQ

Download         Length: 405 bp

>T101346 NZ_CP028490:3298801-3299205 [Pseudomonas syringae pv. atrofaciens]
ATGCTCAAGTACATGCTTGACACCAACATTTGCATTTTCACTATAAAGAACAAGCCAACTTCGGTGCGTGAAGCCTTCAA
CCTCCATCACGGACAGCTATGTATCAGCGCGATCACGTTGATGGAGCTTGTCTACGGCGCAGAAAAGTCTTTGAGCCCTG
AAAGAAACCTTGCCGTTGTAGAGGGTTTCACAGCGCGTCTTGAAGTGCTCCCTTACGACAGCGACGCTGCGGCTCATACC
GGCATGATCAGAGCCGAACTGGCTAGATCGAGCACACCGATCGGCCCGTACGATCAAATGATTGCAGGACACGCCCGCTC
TCTAGGCCTGGTGGTGATAACTAATAATCAGCGTGAATTCCGACGCGTCGAGGGGCTACGAGTAGAAGACTGGGTAAGCC
AGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8508.52 Da        Isoelectric Point: 4.7247

>AT101346 WP_003421652.1 NZ_CP028490:3298571-3298801 [Pseudomonas syringae pv. atrofaciens]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAERGQPAEQQRESL

Download         Length: 231 bp

>AT101346 NZ_CP028490:3298571-3298801 [Pseudomonas syringae pv. atrofaciens]
ATGGAACAATCCACAGTCTTCAAAAGCAATCGAAGCCAGGCTGTCCGACTGCCAAAAGCGGTGGCCTTGCCAGACGACGT
GAAACGTGTCGATGTCGTAGCCGTGGGCAGAACCCGCATTATTTCCCCCGCTGGTGAAATGTGGAACAGCTGGTTCGATG
GTGAAAGTGTCAGTGATGATTTCATGGCAGAGCGCGGACAACCAGCCGAACAGCAGCGCGAATCGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P9KYQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9J133

References