Detailed information of TA system TA101338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2991258..2991892
• Replicon: chromosome
• Accession: NZ_CP028487
• Organism: Yersinia massiliensis strain GTA

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: DA391_RS14045
• Protein ID: WP_050879015.1
• Coordinates: 2991716..2991892 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2R4NRG3
• Locus tag: DA391_RS14040
• Protein ID: WP_050879014.1
• Coordinates: 2991258..2991665 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DA391_RS14000	2986877..2987476	-	600	WP_108087880.1	RNA polymerase subunit sigma-70	-
DA391_RS14005	2987587..2988111	-	525	WP_108087881.1	HNH endonuclease	-
DA391_RS14010	2988188..2988832	-	645	WP_108087882.1	hypothetical protein	-
DA391_RS14015	2989065..2989343	-	279	WP_145566229.1	hypothetical protein	-
DA391_RS14020	2989362..2989772	-	411	WP_145566227.1	hypothetical protein	-
DA391_RS14025	2989783..2990304	-	522	WP_108087885.1	DUF2514 family protein	-
DA391_RS14030	2990297..2990827	-	531	WP_108087886.1	lysozyme	-
DA391_RS14035	2990855..2991070	-	216	WP_050081311.1	phage holin family protein	-
DA391_RS14040	2991258..2991665	-	408	WP_050879014.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DA391_RS14045	2991716..2991892	-	177	WP_050879015.1	type II toxin-antitoxin system HicA family toxin	Toxin
DA391_RS14050	2992577..2992777	-	201	WP_019210745.1	hypothetical protein	-
DA391_RS14055	2993110..2993511	-	402	WP_108087887.1	antitermination protein	-
DA391_RS14060	2993699..2994715	-	1017	WP_108087888.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2963110..3011104	47994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.87 Da
• Isoelectric Point: 10.8471

>T101338 WP_050879015.1 NZ_CP028487:c2991892-2991716 [Yersinia massiliensis]
VKQSEFRRWLEAQGVEIKNGTNHLKLYYQGKQSVMPRHPSKELGESLRKAIIKQLGIK

Nucleotide

• Length: 177 bp

>T101338 NZ_CP028487:c2991892-2991716 [Yersinia massiliensis]
GTGAAGCAGAGCGAGTTTCGAAGATGGCTCGAAGCTCAAGGGGTAGAGATTAAGAACGGAACGAATCATCTTAAACTCTA
CTACCAAGGCAAGCAGTCAGTGATGCCAAGACACCCCAGTAAAGAGTTAGGGGAATCGTTAAGGAAAGCGATAATCAAAC
AGTTAGGCATCAAATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14923.02 Da
• Isoelectric Point: 4.4202

>AT101338 WP_050879014.1 NZ_CP028487:c2991665-2991258 [Yersinia massiliensis]
MRYPVKLQKDGDGYFVSFPDIPEALTQGETREEALEMARDALVTAFDFYFEDNRPVPLPSDSGDDLVSVPASVWAKVLLL
NTMLETGITNAELARRMGLKPQEVQRIVTLGHNTKIDTVEAALTALGKRLELSVS

Nucleotide

• Length: 408 bp

>AT101338 NZ_CP028487:c2991665-2991258 [Yersinia massiliensis]
ATGCGATATCCAGTAAAACTACAGAAAGATGGTGATGGGTACTTTGTTAGTTTCCCAGATATCCCAGAGGCATTAACTCA
GGGCGAAACCAGAGAAGAAGCGTTAGAGATGGCGCGTGACGCTCTGGTAACGGCGTTTGATTTTTACTTCGAAGATAATC
GCCCGGTTCCACTGCCAAGTGATTCGGGTGATGACTTAGTGTCAGTTCCTGCGAGTGTGTGGGCAAAGGTTTTACTGCTC
AACACAATGCTTGAGACCGGAATAACTAACGCTGAATTAGCGCGCCGAATGGGGCTTAAGCCACAGGAGGTTCAGCGGAT
TGTCACGTTAGGCCATAACACAAAGATTGATACAGTCGAGGCAGCGCTTACTGCTCTGGGTAAGCGTCTTGAGCTATCAG
TTAGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A2R4NRG3