Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 77495..78096 Replicon plasmid p1
Accession NZ_CP028484
Organism Escherichia coli strain E41-1

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag C2U19_RS26645 Protein ID WP_001216034.1
Coordinates 77495..77875 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag C2U19_RS26650 Protein ID WP_001190712.1
Coordinates 77875..78096 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U19_RS26605 72546..73478 - 933 WP_110204514.1 homocysteine S-methyltransferase -
C2U19_RS26610 73465..74868 - 1404 WP_001373486.1 S-methylmethionine permease -
C2U19_RS26620 75112..76092 + 981 WP_000019407.1 IS5-like element IS5 family transposase -
C2U19_RS26625 76320..76637 + 318 WP_001513659.1 hypothetical protein -
C2U19_RS26630 76587..77000 - 414 Protein_91 DDE-type integrase/transposase/recombinase -
C2U19_RS26635 76924..77283 - 360 WP_001513660.1 hypothetical protein -
C2U19_RS26640 77311..77490 - 180 WP_001513661.1 hypothetical protein -
C2U19_RS26645 77495..77875 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
C2U19_RS26650 77875..78096 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
C2U19_RS26655 78279..79835 + 1557 WP_001617892.1 type I restriction-modification system subunit M -
C2U19_RS26660 79832..81115 + 1284 WP_001617890.1 restriction endonuclease subunit S -
C2U19_RS26665 81237..82553 + 1317 Protein_98 HsdR family type I site-specific deoxyribonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17 senB 1..128911 128911


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T101314 WP_001216034.1 NZ_CP028484:c77875-77495 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T101314 NZ_CP028484:c77875-77495 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT101314 WP_001190712.1 NZ_CP028484:c78096-77875 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT101314 NZ_CP028484:c78096-77875 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References