Detailed information of TA system TA101305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4262396..4263216
• Replicon: chromosome
• Accession: NZ_CP028483
• Organism: Escherichia coli strain E41-1

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: C2U19_RS22195
• Protein ID: WP_001054379.1
• Coordinates: 4262396..4262653 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: C2U19_RS22200
• Protein ID: WP_000123957.1
• Coordinates: 4262665..4263216 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U19_RS22170	4257943..4258923	+	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
C2U19_RS22175	4259506..4260612	-	1107	Protein_4080	helicase YjhR	-
C2U19_RS22180	4260675..4261822	+	1148	WP_085949154.1	IS3-like element ISEc52 family transposase	-
C2U19_RS22185	4261855..4262019	+	165	Protein_4082	GNAT family N-acetyltransferase	-
C2U19_RS22195	4262396..4262653	+	258	WP_001054379.1	YjhX family toxin	Toxin
C2U19_RS22200	4262665..4263216	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
C2U19_RS22205	4263268..4264014	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
C2U19_RS22210	4264142..4264402	+	261	WP_000077645.1	hypothetical protein	-
C2U19_RS28305	4264440..4264556	+	117	Protein_4087	VOC family protein	-
C2U19_RS22215	4264801..4265922	+	1122	WP_000010833.1	M42 family metallopeptidase	-
C2U19_RS22220	4265919..4266197	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
C2U19_RS22225	4266209..4267522	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE	4251017..4271437	20420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T101305 WP_001054379.1 NZ_CP028483:4262396-4262653 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T101305 NZ_CP028483:4262396-4262653 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT101305 WP_000123957.1 NZ_CP028483:4262665-4263216 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT101305 NZ_CP028483:4262665-4263216 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030