Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3649020..3649638 Replicon chromosome
Accession NZ_CP028483
Organism Escherichia coli strain E41-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C2U19_RS19095 Protein ID WP_001291435.1
Coordinates 3649420..3649638 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C2U19_RS19090 Protein ID WP_000344800.1
Coordinates 3649020..3649394 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U19_RS19080 3644109..3645302 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C2U19_RS19085 3645325..3648474 + 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
C2U19_RS19090 3649020..3649394 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C2U19_RS19095 3649420..3649638 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C2U19_RS19100 3649812..3650363 + 552 WP_000102539.1 maltose O-acetyltransferase -
C2U19_RS19105 3650479..3650949 + 471 WP_000136192.1 YlaC family protein -
C2U19_RS19110 3651113..3652663 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C2U19_RS19115 3652705..3653058 - 354 WP_000878135.1 DUF1428 family protein -
C2U19_RS19125 3653437..3653748 + 312 WP_000409908.1 MGMT family protein -
C2U19_RS19130 3653779..3654351 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T101301 WP_001291435.1 NZ_CP028483:3649420-3649638 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T101301 NZ_CP028483:3649420-3649638 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT101301 WP_000344800.1 NZ_CP028483:3649020-3649394 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT101301 NZ_CP028483:3649020-3649394 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References