Detailed information of TA system TA101283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2665840..2666603
• Replicon: chromosome
• Accession: NZ_CP028481
• Organism: Vibrio parahaemolyticus strain S107-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A366A926
• Locus tag: DA442_RS13605
• Protein ID: WP_045595200.1
• Coordinates: 2666097..2666603 (+)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: DA442_RS13600
• Protein ID: WP_000212004.1
• Coordinates: 2665840..2666106 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DA442_RS13585	2662749..2663441	-	693	WP_134990653.1	DsbC family protein	-
DA442_RS13590	2663783..2664721	+	939	WP_167733321.1	Abi family protein	-
DA442_RS13595	2665149..2665685	+	537	WP_134990655.1	hypothetical protein	-
DA442_RS13600	2665840..2666106	+	267	WP_000212004.1	DUF1778 domain-containing protein	Antitoxin
DA442_RS13605	2666097..2666603	+	507	WP_045595200.1	GNAT family N-acetyltransferase	Toxin
DA442_RS13610	2666644..2667030	-	387	WP_004249359.1	hypothetical protein	-
DA442_RS13615	2667027..2667599	-	573	WP_167733322.1	type IV conjugative transfer system lipoprotein TraV	-
DA442_RS13620	2667674..2668963	-	1290	WP_134990657.1	TraB/VirB10 family protein	-
DA442_RS13625	2668966..2669862	-	897	WP_134990708.1	type-F conjugative transfer system secretin TraK	-
DA442_RS13630	2669846..2670472	-	627	WP_134990658.1	pilus assembly protein	-
DA442_RS13635	2670469..2670750	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
DA442_RS13640	2670984..2671274	+	291	WP_134990659.1	HigA family addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2665149..2696895	31746

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-83)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18277.06 Da
• Isoelectric Point: 7.2134

>T101283 WP_045595200.1 NZ_CP028481:2666097-2666603 [Vibrio parahaemolyticus]
MGISAPTLLTADHEINAFQCGIEELDTWLRKQALKSQKRGTARVYVVNDTEVHQVIGYYAIAMGSVSREQAFSSLRRNSP
DPIPMVILARLGVDNAYQGQGIAAGLLKDCIIRSVQAMNAVGGAGILVHAIDGSAQTFYKKFGFKESTFDPLVLMARICD
IEKSLSID

Nucleotide

• Length: 507 bp

>T101283 NZ_CP028481:2666097-2666603 [Vibrio parahaemolyticus]
TTGGGAATAAGCGCACCAACCCTACTCACTGCTGATCACGAGATTAACGCCTTTCAATGTGGCATCGAAGAACTCGATAC
TTGGTTAAGAAAGCAGGCGTTAAAAAGTCAAAAGCGGGGTACCGCGAGAGTGTATGTGGTCAATGACACTGAAGTCCATC
AAGTGATTGGGTACTATGCCATTGCCATGGGATCAGTTTCCAGAGAGCAAGCTTTCAGTTCATTAAGAAGAAATAGTCCA
GATCCCATTCCCATGGTGATTCTAGCAAGGCTTGGGGTAGATAACGCCTATCAAGGTCAAGGCATTGCGGCAGGTCTTTT
AAAAGATTGTATCATTCGCTCAGTACAAGCAATGAACGCTGTTGGTGGAGCAGGAATCCTAGTCCATGCTATTGATGGTA
GTGCTCAAACGTTCTACAAGAAATTTGGCTTCAAAGAATCGACATTTGATCCTTTAGTACTCATGGCAAGGATCTGCGAT
ATCGAAAAATCATTGTCGATAGACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9956.48 Da
• Isoelectric Point: 5.8580

>AT101283 WP_000212004.1 NZ_CP028481:2665840-2666106 [Vibrio parahaemolyticus]
MATKTAPINMRVLPSVRDIIDAAASLKKVDRTVFIQQAALNEAHSILAEQRDFVLEAKAFEAFDNELRAEPQTLEGMKDL
FKRKAPWE

Nucleotide

• Length: 267 bp

>AT101283 NZ_CP028481:2665840-2666106 [Vibrio parahaemolyticus]
ATGGCTACAAAGACAGCCCCAATCAACATGCGAGTACTGCCATCGGTAAGAGACATCATCGATGCCGCAGCAAGTTTAAA
AAAAGTTGATAGAACCGTGTTCATTCAGCAAGCAGCGCTCAATGAAGCGCATAGTATACTGGCAGAACAGCGGGATTTTG
TTCTAGAAGCAAAGGCTTTTGAAGCGTTTGATAACGAACTTCGTGCAGAGCCACAGACTCTTGAAGGTATGAAAGATCTG
TTTAAAAGGAAAGCGCCTTGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A366A926