Detailed information of TA system TA101273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4658123..4658639
• Replicon: chromosome
• Accession: NZ_CP028478
• Organism: Klebsiella pneumoniae strain 2e

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DA795_RS23075
• Protein ID: WP_122051597.1
• Coordinates: 4658123..4658407 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: DA795_RS23080
• Protein ID: WP_002886901.1
• Coordinates: 4658397..4658639 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DA795_RS23055	4653599..4653907	-	309	WP_040172617.1	PTS sugar transporter subunit IIB	-
DA795_RS28085	4653992..4654165	+	174	WP_040248667.1	hypothetical protein	-
DA795_RS23060	4654168..4654911	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
DA795_RS23065	4655268..4657406	+	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
DA795_RS23070	4657655..4658119	+	465	WP_004178375.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
DA795_RS23075	4658123..4658407	-	285	WP_122051597.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DA795_RS23080	4658397..4658639	-	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DA795_RS23085	4658717..4660627	-	1911	WP_032412857.1	BglG family transcription antiterminator	-
DA795_RS23090	4660650..4661804	-	1155	WP_004178372.1	lactonase family protein	-
DA795_RS23095	4661871..4662611	-	741	WP_002886899.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11186.04 Da
• Isoelectric Point: 10.3787

>T101273 WP_122051597.1 NZ_CP028478:c4658407-4658123 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNLRIESTRLSDLPDCYKIKLKASGYRLLYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T101273 NZ_CP028478:c4658407-4658123 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGTTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT101273 WP_002886901.1 NZ_CP028478:c4658639-4658397 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT101273 NZ_CP028478:c4658639-4658397 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLP0