Detailed information of TA system TA101249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2075739..2076268
• Replicon: chromosome
• Accession: NZ_CP028470
• Organism: Staphylococcus aureus strain IT4-R

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C5Y81_RS10610
• Protein ID: WP_000621175.1
• Coordinates: 2075739..2076101 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C5Y81_RS10615
• Protein ID: WP_000948330.1
• Coordinates: 2076098..2076268 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5Y81_RS10585	2072718..2073488	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
C5Y81_RS10590	2073463..2073942	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
C5Y81_RS10595	2073944..2074270	-	327	WP_001052491.1	anti-sigma factor antagonist	-
C5Y81_RS10600	2074390..2075391	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
C5Y81_RS10610	2075739..2076101	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C5Y81_RS10615	2076098..2076268	-	171	WP_000948330.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
C5Y81_RS10620	2076353..2077501	-	1149	WP_001281141.1	alanine racemase	-
C5Y81_RS10625	2077567..2077926	-	360	WP_000581193.1	holo-ACP synthase	-
C5Y81_RS10630	2077930..2078421	-	492	WP_001205918.1	PH domain-containing protein	-
C5Y81_RS10635	2078408..2079991	-	1584	WP_001294624.1	PH domain-containing protein	-
C5Y81_RS10640	2079984..2080463	-	480	WP_001287080.1	hypothetical protein	-
C5Y81_RS10645	2080672..2081232	-	561	WP_001092400.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T101249 WP_000621175.1 NZ_CP028470:c2076101-2075739 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T101249 NZ_CP028470:c2076101-2075739 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATTATTCAAAATGACACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAATATAAATTGGATAAAGACTCAGTCATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTATTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6237.75 Da
• Isoelectric Point: 3.8305

>AT101249 WP_000948330.1 NZ_CP028470:c2076268-2076098 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNESYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT101249 NZ_CP028470:c2076268-2076098 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAATCATATTTATCTTCTAATTCAA
CGAATGAATGA