Detailed information of TA system TA101234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2095495..2096009
• Replicon: chromosome
• Accession: NZ_CP028468
• Organism: Staphylococcus aureus strain IT1-S

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: C5Y45_RS10700
• Protein ID: WP_000581792.1
• Coordinates: 2095495..2095704 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: C5Y45_RS10705
• Protein ID: WP_000138928.1
• Coordinates: 2095716..2096009 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5Y45_RS10680	2091003..2092361	-	1359	WP_000611469.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
C5Y45_RS10685	2092376..2093446	-	1071	WP_000159632.1	D-alanine--D-alanine ligase	-
C5Y45_RS10690	2093764..2094966	+	1203	WP_001109944.1	rod shape-determining protein RodA	-
C5Y45_RS10695	2095230..2095367	-	138	WP_000828354.1	hypothetical protein	-
C5Y45_RS10700	2095495..2095704	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
C5Y45_RS10705	2095716..2096009	-	294	WP_000138928.1	copper-sensing transcriptional repressor CsoR	Antitoxin
C5Y45_RS10710	2096175..2097659	+	1485	WP_000571551.1	cardiolipin synthase	-
C5Y45_RS10715	2097687..2098334	+	648	WP_001187617.1	HD domain-containing protein	-
C5Y45_RS10725	2098812..2099684	-	873	WP_000725802.1	membrane protein insertase YidC	-
C5Y45_RS10730	2099770..2100411	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T101234 WP_000581792.1 NZ_CP028468:c2095704-2095495 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T101234 NZ_CP028468:c2095704-2095495 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11184.82 Da
• Isoelectric Point: 6.2243

>AT101234 WP_000138928.1 NZ_CP028468:c2096009-2095716 [Staphylococcus aureus]
MTEQDIAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT101234 NZ_CP028468:c2096009-2095716 [Staphylococcus aureus]
ATGACTGAACAAGATATTGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1