Detailed information of TA system TA101229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1854681..1855457
• Replicon: chromosome
• Accession: NZ_CP028468
• Organism: Staphylococcus aureus strain IT1-S

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: C5Y45_RS09180
• Protein ID: WP_000031112.1
• Coordinates: 1854681..1854833 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: C5Y45_RS09185
• Protein ID: WP_001251224.1
• Coordinates: 1854858..1855457 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5Y45_RS09165	1850647..1851468	+	822	WP_000669382.1	RluA family pseudouridine synthase	-
C5Y45_RS09170	1851931..1853316	-	1386	WP_110164473.1	class II fumarate hydratase	-
C5Y45_RS09175	1853512..1853907	-	396	WP_000901023.1	hypothetical protein	-
C5Y45_RS14500	1854208..1854348	+	141	WP_153172984.1	hypothetical protein	-
C5Y45_RS09180	1854681..1854833	-	153	WP_000031112.1	hypothetical protein	Toxin
C5Y45_RS09185	1854858..1855457	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
C5Y45_RS09190	1855616..1856086	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
C5Y45_RS09195	1856091..1857218	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
C5Y45_RS09200	1857368..1858090	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
C5Y45_RS09205	1858083..1859540	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5966.31 Da
• Isoelectric Point: 3.8962

>T101229 WP_000031112.1 NZ_CP028468:c1854833-1854681 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELSKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T101229 NZ_CP028468:c1854833-1854681 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAAGTAAAGAAATGGAACAAATTTCCGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT101229 WP_001251224.1 NZ_CP028468:c1855457-1854858 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT101229 NZ_CP028468:c1855457-1854858 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: W8U4V4