Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 36267..36904 Replicon chromosome
Accession NZ_CP028441
Organism Bacillus velezensis strain 131-4

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag DA379_RS00220 Protein ID WP_003156187.1
Coordinates 36554..36904 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag DA379_RS00215 Protein ID WP_003156188.1
Coordinates 36267..36548 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DA379_RS00195 32632..33231 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
DA379_RS00200 33324..33689 + 366 WP_003156192.1 holo-ACP synthase -
DA379_RS00205 33854..34861 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
DA379_RS00210 34978..36147 + 1170 WP_003156189.1 alanine racemase -
DA379_RS00215 36267..36548 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
DA379_RS00220 36554..36904 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DA379_RS00225 37022..37843 + 822 WP_003156182.1 STAS domain-containing protein -
DA379_RS00230 37848..38213 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
DA379_RS00235 38216..38617 + 402 WP_003156178.1 anti-sigma regulatory factor -
DA379_RS00240 38629..39636 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
DA379_RS00245 39700..40029 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
DA379_RS00250 40026..40508 + 483 WP_003156173.1 anti-sigma B factor RsbW -
DA379_RS00255 40474..41262 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
DA379_RS00260 41262..41864 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T101224 WP_003156187.1 NZ_CP028441:36554-36904 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T101224 NZ_CP028441:36554-36904 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT101224 WP_003156188.1 NZ_CP028441:36267-36548 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT101224 NZ_CP028441:36267-36548 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References