Detailed information of TA system TA101223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1915597..1916234
• Replicon: chromosome
• Accession: NZ_CP028440
• Organism: Bacillus velezensis strain J7-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: DA378_RS09150
• Protein ID: WP_003156187.1
• Coordinates: 1915597..1915947 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: DA378_RS09155
• Protein ID: WP_003156188.1
• Coordinates: 1915953..1916234 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DA378_RS09110	1910637..1911239	-	603	WP_003156170.1	SpoIIE family protein phosphatase	-
DA378_RS09115	1911239..1912027	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
DA378_RS09120	1911993..1912475	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
DA378_RS09125	1912472..1912801	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
DA378_RS09130	1912865..1913872	-	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
DA378_RS09135	1913884..1914285	-	402	WP_003156178.1	anti-sigma regulatory factor	-
DA378_RS09140	1914288..1914653	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
DA378_RS09145	1914658..1915479	-	822	WP_003156182.1	STAS domain-containing protein	-
DA378_RS09150	1915597..1915947	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
DA378_RS09155	1915953..1916234	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
DA378_RS09160	1916354..1917523	-	1170	WP_003156189.1	alanine racemase	-
DA378_RS09165	1917640..1918647	-	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
DA378_RS09170	1918812..1919177	-	366	WP_003156192.1	holo-ACP synthase	-
DA378_RS09175	1919270..1919869	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T101223 WP_003156187.1 NZ_CP028440:c1915947-1915597 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T101223 NZ_CP028440:c1915947-1915597 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT101223 WP_003156188.1 NZ_CP028440:c1916234-1915953 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT101223 NZ_CP028440:c1916234-1915953 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5