Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1131086..1132003 Replicon chromosome
Accession NZ_CP028440
Organism Bacillus velezensis strain J7-1

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag DA378_RS04885 Protein ID WP_003154806.1
Coordinates 1131086..1131832 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag DA378_RS04890 Protein ID WP_003154807.1
Coordinates 1131833..1132003 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DA378_RS04865 1126478..1127428 - 951 WP_003154799.1 ring-cleaving dioxygenase -
DA378_RS04870 1127751..1129067 + 1317 WP_003154801.1 amino acid permease -
DA378_RS04875 1129353..1129970 + 618 WP_003154804.1 DUF47 domain-containing protein -
DA378_RS04880 1129983..1130981 + 999 WP_003154805.1 inorganic phosphate transporter -
DA378_RS04885 1131086..1131832 + 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
DA378_RS04890 1131833..1132003 + 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
DA378_RS04895 1132261..1133139 - 879 WP_003154811.1 N-acetylmuramoyl-L-alanine amidase -
DA378_RS04900 1133153..1133416 - 264 WP_003154813.1 phage holin -
DA378_RS04905 1133430..1133693 - 264 WP_003154815.1 hemolysin XhlA family protein -
DA378_RS04910 1133745..1134506 - 762 WP_003154817.1 hypothetical protein -
DA378_RS04915 1134563..1134760 - 198 WP_003154819.1 XkdX family protein -
DA378_RS04920 1134766..1135137 - 372 WP_003154820.1 hypothetical protein -
DA378_RS04925 1135150..1136772 - 1623 WP_003154821.1 pyocin knob domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T101222 WP_003154806.1 NZ_CP028440:1131086-1131832 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T101222 NZ_CP028440:1131086-1131832 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTTCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT101222 WP_003154807.1 NZ_CP028440:1131833-1132003 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT101222 NZ_CP028440:1131833-1132003 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGATAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References