Detailed information of TA system TA101221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3604286..3604923
• Replicon: chromosome
• Accession: NZ_CP028439
• Organism: Bacillus velezensis strain 8-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: DA377_RS18125
• Protein ID: WP_003156187.1
• Coordinates: 3604286..3604636 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: DA377_RS18130
• Protein ID: WP_003156188.1
• Coordinates: 3604642..3604923 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DA377_RS18085	3599326..3599928	-	603	WP_003156170.1	SpoIIE family protein phosphatase	-
DA377_RS18090	3599928..3600716	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
DA377_RS18095	3600682..3601164	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
DA377_RS18100	3601161..3601490	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
DA377_RS18105	3601554..3602561	-	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
DA377_RS18110	3602573..3602974	-	402	WP_003156178.1	anti-sigma regulatory factor	-
DA377_RS18115	3602977..3603342	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
DA377_RS18120	3603347..3604168	-	822	WP_003156182.1	STAS domain-containing protein	-
DA377_RS18125	3604286..3604636	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
DA377_RS18130	3604642..3604923	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
DA377_RS18135	3605043..3606212	-	1170	WP_003156189.1	alanine racemase	-
DA377_RS18140	3606329..3607336	-	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
DA377_RS18145	3607501..3607866	-	366	WP_003156192.1	holo-ACP synthase	-
DA377_RS18150	3607959..3608558	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T101221 WP_003156187.1 NZ_CP028439:c3604636-3604286 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T101221 NZ_CP028439:c3604636-3604286 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT101221 WP_003156188.1 NZ_CP028439:c3604923-3604642 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT101221 NZ_CP028439:c3604923-3604642 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5