Detailed information of TA system TA101218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 508006..508643
• Replicon: chromosome
• Accession: NZ_CP028437
• Organism: Bacillus velezensis strain DR-08

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: DA376_RS02640
• Protein ID: WP_003156187.1
• Coordinates: 508293..508643 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: DA376_RS02635
• Protein ID: WP_003156188.1
• Coordinates: 508006..508287 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DA376_RS02615	504371..504970	-	600	WP_032872997.1	rhomboid family intramembrane serine protease	-
DA376_RS02620	505063..505428	+	366	WP_007609580.1	holo-ACP synthase	-
DA376_RS02625	505593..506600	+	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
DA376_RS02630	506717..507886	+	1170	WP_032873001.1	alanine racemase	-
DA376_RS02635	508006..508287	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
DA376_RS02640	508293..508643	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
DA376_RS02645	508761..509582	+	822	WP_003156182.1	STAS domain-containing protein	-
DA376_RS02650	509587..509952	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
DA376_RS02655	509955..510356	+	402	WP_003156178.1	anti-sigma regulatory factor	-
DA376_RS02660	510368..511375	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
DA376_RS02665	511439..511768	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
DA376_RS02670	511765..512247	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
DA376_RS02675	512213..513001	+	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
DA376_RS02680	513001..513603	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T101218 WP_003156187.1 NZ_CP028437:508293-508643 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T101218 NZ_CP028437:508293-508643 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT101218 WP_003156188.1 NZ_CP028437:508006-508287 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT101218 NZ_CP028437:508006-508287 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5