Detailed information of TA system TA101213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 41090..41888
• Replicon: plasmid pRM9975-1
• Accession: NZ_CP028433
• Organism: Escherichia coli strain RM9975

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: ECRM9975_RS27995
• Protein ID: WP_112033868.1
• Coordinates: 41367..41888 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0V9R6Q7
• Locus tag: ECRM9975_RS27990
• Protein ID: WP_001351987.1
• Coordinates: 41090..41359 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM9975_RS27965	36588..37328	-	741	WP_001717320.1	hypothetical protein	-
ECRM9975_RS27970	37611..38378	+	768	WP_000342417.1	hypothetical protein	-
ECRM9975_RS27975	38431..38784	-	354	WP_160378290.1	hypothetical protein	-
ECRM9975_RS27980	38790..39458	-	669	WP_000161228.1	AAA family ATPase	-
ECRM9975_RS27985	39634..40847	+	1214	WP_162829202.1	IS3 family transposase	-
ECRM9975_RS27990	41090..41359	+	270	WP_001351987.1	DUF1778 domain-containing protein	Antitoxin
ECRM9975_RS27995	41367..41888	+	522	WP_112033868.1	GNAT family N-acetyltransferase	Toxin
ECRM9975_RS28005	42057..42308	-	252	WP_000901559.1	hypothetical protein	-
ECRM9975_RS28010	42310..43002	-	693	WP_012640731.1	hypothetical protein	-
ECRM9975_RS28015	43016..43339	-	324	WP_000064175.1	hypothetical protein	-
ECRM9975_RS28020	43542..46205	-	2664	WP_112033869.1	tail fiber protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..120253	120253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19402.21 Da
• Isoelectric Point: 8.6369

>T101213 WP_112033868.1 NZ_CP028433:41367-41888 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKPLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T101213 NZ_CP028433:41367-41888 [Escherichia coli]
GTGAGCAATACAACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGCCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGCACTCAAGAAG
AAAGGCCAAAAGTGTTAGGATATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCAAGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGCCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10082.54 Da
• Isoelectric Point: 7.0155

>AT101213 WP_001351987.1 NZ_CP028433:41090-41359 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Nucleotide

• Length: 270 bp

>AT101213 NZ_CP028433:41090-41359 [Escherichia coli]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0V9R6Q7