Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mosAT/AbiEii-AbiEi
Location 5109465..5111148 Replicon chromosome
Accession NZ_CP028432
Organism Escherichia coli strain RM9975

Toxin (Protein)


Gene name mosT Uniprot ID I2UPR9
Locus tag ECRM9975_RS27115 Protein ID WP_000361917.1
Coordinates 5109465..5110382 (-) Length 306 a.a.

Antitoxin (Protein)


Gene name mosA Uniprot ID I2UPX5
Locus tag ECRM9975_RS27120 Protein ID WP_000157137.1
Coordinates 5110375..5111148 (-) Length 258 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM9975_RS27090 5106075..5107094 - 1020 WP_001345322.1 NADPH-dependent aldehyde reductase Ahr -
ECRM9975_RS27105 5107575..5108285 + 711 WP_001219055.1 integrase arm-type DNA-binding domain-containing protein -
ECRM9975_RS27110 5108289..5108951 + 663 WP_000357928.1 hypothetical protein -
ECRM9975_RS29560 5109004..5109171 - 168 WP_001356018.1 hypothetical protein -
ECRM9975_RS27115 5109465..5110382 - 918 WP_000361917.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
ECRM9975_RS27120 5110375..5111148 - 774 WP_000157137.1 type IV toxin-antitoxin system AbiEi family antitoxin Antitoxin
ECRM9975_RS27125 5111232..5111552 + 321 Protein_4953 IS3 family transposase -
ECRM9975_RS27130 5111728..5113077 - 1350 WP_000766442.1 phospholipase -
ECRM9975_RS27135 5113196..5115280 - 2085 WP_001193075.1 protease Lon-related BREX system protein BrxL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(13-278)

Antitoxin

Transcriptional regulator AbiEi antitoxin, N-terminal domain

(1-93)


Sequences

Toxin        


Download         Length: 306 a.a.        Molecular weight: 34963.04 Da        Isoelectric Point: 4.7716

>T101212 WP_000361917.1 NZ_CP028432:c5110382-5109465 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELEAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Download         Length: 918 bp

>T101212 NZ_CP028432:c5110382-5109465 [Escherichia coli]
ATGGATAAAACCTCCCCCTACTACCGCCAGGTCGCTCTGTTGGTGCGAGCATTACCCTGGGTCGCTAACGAGTCCTGTTT
TGCGCTGAAAGGCGGAACGGCGATCAACTTATTCATCCATGATTTTCCCCGATTGTCCGTTGATATCGATCTGGCCTGGA
TACCGCTGGAAAGCAGAGAGGTCGCGCTGCCCAACGTGCGGGAGGCGCTCACCCGTATCGCCGCAGAACTGGAAGCGCAA
ACCGGGATCCGTGCAGTGCTGCAAACCCATAATCCCGATGAGATGCGCATCATTGTCACCACTGACGATGCGCAAATTAA
AATTGAAGTCTCGCCAGTTGCCCGGGGGACACTATATCCTCCGCAAGAACGCGATATCGTCGGAGCCGTGGAGGAAGAAT
TTGGCTTTGCCGCGCTCCCGGTGGTCTCACTGCCCGATTTATACGGTGGCAAGCTATGTGCTGCGTTAGATCGCCAGCAC
CCCAGAGATTTTTACGACGTCAAAATGCTCCTTGATACACAAGGAATTGATCGCTCGATATTCAATGGTTTTATCGTGTA
CTTGCTCAGCCATAACCGCCCATTATCCGAAGTGCTTAACCCACGTTGGAAGGATATTACTGAACCCTTTCAAAATGAAT
TCAGCGGTATGACTTTTGAGGAAGTATCACTGGAAGAGCTAAAAGAGGTCCCGGAAAGAATGCTCAGCACGTTGAAAACG
CATTTCACTCAACAGGATTTCGACTTCCTGATGTCATTTAAACGCGGCGAGCCAGACTGGAATTTAGCGCCAGAGCCCCA
CATTCAGAATTTGCCAGCCGTTCAATGGAAGCTGCGCAATATTGGACAGATGTCGGAGGAAAAGCGCGCGCAGTCACTGG
AGAAACTAGAAAAGGTCATGACAGAATGGCTACGTTAG

Antitoxin


Download         Length: 258 a.a.        Molecular weight: 29333.76 Da        Isoelectric Point: 9.9889

>AT101212 WP_000157137.1 NZ_CP028432:c5111148-5110375 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Download         Length: 774 bp

>AT101212 NZ_CP028432:c5111148-5110375 [Escherichia coli]
ATGGCATCAAAATTAAACTGGCTGTTGCAAAACACCTCTCCGGGAAGCCTGGTGTTACAATCGTGGCTGACCCGGAACAA
CATATCACCTTCTCTGGCTTTTAAATACGCGCAAAATGGCTGGTTAATAAAACTGCGGGCTGGCGTTTATGCGCGCGCAG
GACGAGAACCAGACTGGAGTGATGCGCTATGGTGCCTACAAAACCAACTTGAAGCGCCGGTGCATCTGGCCGGACTGAGT
AGTCTCTCCTGGCAGGGCCGTTCCCATTACCTACAACTTAAGCAGAACCAATGCTGGCTTAGCGTTGAAAACAAAGCGAT
ATTGCCAAAATGGTTTAAAGAATTTCCCGCCGTTGAGTGGTTGATGGTATCGGCCCTGAAGCTCCCTGACCTGGATGAAA
AATACCGAACTGAGCTTGAGGTTAAAGGGAAAAGAATTACCGCCAGCACGCCAGAACTGGCTGCCTATGAGCTGTTAAGC
GCAGTCCCTCAGGAAATCTCATTTGAGCACGCTGCCGAGCTTTTTCAGGGACTAGTCAATCTCAACCCACGTAAAGTGGA
GAAGCTGCTTACACTCAGTCAGTCCGTACAAACCAAGCGCCTGTATCTGTTTTTTGCCAGTTTCTATGAACATGCATGGT
TCAAACGCATAGATACCAGCAAAATCAACCTGGGATCGGGCAACCGACAAATCGTTGTTAATGGCAAGCTGAACACGCAA
TACCAGATAACTGTGCCCGAAAATTTTACTAAGAAGGGAATAAGCCATGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0PS08

Antitoxin

Source ID Structure
AlphaFold DB A0A0H0N3W2

References