Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 5064680..5065275 Replicon chromosome
Accession NZ_CP028432
Organism Escherichia coli strain RM9975

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag ECRM9975_RS26895 Protein ID WP_000239579.1
Coordinates 5064925..5065275 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag ECRM9975_RS26890 Protein ID WP_001223208.1
Coordinates 5064680..5064931 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM9975_RS26880 5060345..5064124 + 3780 WP_000060925.1 autotransporter assembly complex protein TamB -
ECRM9975_RS26885 5064127..5064468 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
ECRM9975_RS26890 5064680..5064931 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
ECRM9975_RS26895 5064925..5065275 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
ECRM9975_RS26900 5065355..5065885 - 531 WP_000055075.1 inorganic diphosphatase -
ECRM9975_RS26905 5066195..5067151 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
ECRM9975_RS26910 5067291..5068793 + 1503 WP_000205815.1 sugar ABC transporter ATP-binding protein -
ECRM9975_RS26915 5068807..5069829 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T101211 WP_000239579.1 NZ_CP028432:5064925-5065275 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T101211 NZ_CP028432:5064925-5065275 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT101211 WP_001223208.1 NZ_CP028432:5064680-5064931 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT101211 NZ_CP028432:5064680-5064931 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References