Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4945745..4946242 Replicon chromosome
Accession NZ_CP028432
Organism Escherichia coli strain RM9975

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECRM9975_RS26250 Protein ID WP_001173343.1
Coordinates 4946069..4946242 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECRM9975_RS26245 Protein ID WP_000398619.1
Coordinates 4945745..4946041 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM9975_RS26220 4942476..4943195 - 720 WP_000611299.1 two-component system response regulator DcuR -
ECRM9975_RS26225 4943192..4944823 - 1632 WP_001216487.1 sensor histidine kinase -
ECRM9975_RS26230 4945004..4945234 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
ECRM9975_RS26235 4945246..4945518 + 273 WP_000405647.1 N-acetyltransferase -
ECRM9975_RS26245 4945745..4946041 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECRM9975_RS26250 4946069..4946242 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECRM9975_RS26255 4946361..4947101 - 741 Protein_4794 lysine--tRNA ligase -
ECRM9975_RS26260 4947135..4948348 + 1214 WP_162829202.1 IS3 family transposase -
ECRM9975_RS26265 4948384..4948545 - 162 Protein_4796 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T101210 WP_001173343.1 NZ_CP028432:4946069-4946242 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T101210 NZ_CP028432:4946069-4946242 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT101210 WP_000398619.1 NZ_CP028432:4945745-4946041 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT101210 NZ_CP028432:4945745-4946041 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References