Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1351643..1352114 Replicon chromosome
Accession NZ_CP028432
Organism Escherichia coli strain RM9975

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag ECRM9975_RS07115 Protein ID WP_001303511.1
Coordinates 1351643..1351921 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ECRM9975_RS07120 Protein ID WP_001302048.1
Coordinates 1351923..1352114 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM9975_RS07075 1346950..1347219 - 270 WP_000003742.1 excisionase -
ECRM9975_RS07080 1347281..1349746 - 2466 WP_000048442.1 exonuclease -
ECRM9975_RS07085 1349839..1350030 - 192 WP_001090200.1 DUF1482 family protein -
ECRM9975_RS07090 1350027..1350215 - 189 WP_000449192.1 cell division inhibition protein DicB -
ECRM9975_RS07100 1350699..1350917 - 219 WP_001171930.1 hypothetical protein -
ECRM9975_RS07105 1350958..1351347 - 390 WP_001447517.1 hypothetical protein -
ECRM9975_RS07115 1351643..1351921 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECRM9975_RS07120 1351923..1352114 - 192 WP_001302048.1 hypothetical protein Antitoxin
ECRM9975_RS07125 1352135..1352506 - 372 WP_001169687.1 hypothetical protein -
ECRM9975_RS07130 1352604..1352906 + 303 WP_001444689.1 transcriptional regulator -
ECRM9975_RS07135 1352903..1353328 + 426 WP_000693943.1 Rha family transcriptional regulator -
ECRM9975_RS07140 1353351..1354313 + 963 WP_000095671.1 helix-turn-helix domain-containing protein -
ECRM9975_RS07145 1354354..1354764 + 411 Protein_1289 DUF977 family protein -
ECRM9975_RS07155 1356059..1356199 + 141 WP_072058819.1 type I toxin-antitoxin system Hok family toxin -
ECRM9975_RS07165 1356367..1356639 + 273 WP_012817871.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1345863..1390694 44831


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T101188 WP_001303511.1 NZ_CP028432:c1351921-1351643 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T101188 NZ_CP028432:c1351921-1351643 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT101188 WP_001302048.1 NZ_CP028432:c1352114-1351923 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT101188 NZ_CP028432:c1352114-1351923 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References