Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3478860..3479514 Replicon chromosome
Accession NZ_CP028429
Organism Escherichia coli strain RM9131

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECRM9131_RS18610 Protein ID WP_000244777.1
Coordinates 3478860..3479267 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECRM9131_RS18615 Protein ID WP_000354046.1
Coordinates 3479248..3479514 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM9131_RS18590 3474817..3476550 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
ECRM9131_RS18595 3476556..3477266 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECRM9131_RS18600 3477291..3478187 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECRM9131_RS18605 3478299..3478820 + 522 WP_001055874.1 flavodoxin FldB -
ECRM9131_RS18610 3478860..3479267 - 408 WP_000244777.1 toxin CptA Toxin
ECRM9131_RS18615 3479248..3479514 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECRM9131_RS18620 3479757..3480737 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
ECRM9131_RS18625 3480933..3481592 - 660 WP_000250274.1 hemolysin III family protein -
ECRM9131_RS18630 3481756..3482067 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
ECRM9131_RS18635 3482112..3483545 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
ECRM9131_RS18640 3483602..3484345 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T101173 WP_000244777.1 NZ_CP028429:c3479267-3478860 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T101173 NZ_CP028429:c3479267-3478860 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT101173 WP_000354046.1 NZ_CP028429:c3479514-3479248 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT101173 NZ_CP028429:c3479514-3479248 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References