Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3337642..3338225 Replicon chromosome
Accession NZ_CP028429
Organism Escherichia coli strain RM9131

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag ECRM9131_RS17930 Protein ID WP_000254745.1
Coordinates 3337642..3337977 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag ECRM9131_RS17935 Protein ID WP_000581937.1
Coordinates 3337977..3338225 (-) Length 83 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM9131_RS17915 3333529..3334827 - 1299 WP_000036723.1 phosphopyruvate hydratase -
ECRM9131_RS17920 3334915..3336552 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
ECRM9131_RS17925 3336780..3337571 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
ECRM9131_RS17930 3337642..3337977 - 336 WP_000254745.1 endoribonuclease MazF Toxin
ECRM9131_RS17935 3337977..3338225 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
ECRM9131_RS17940 3338303..3340537 - 2235 WP_000226815.1 GTP pyrophosphokinase -
ECRM9131_RS17945 3340585..3341885 - 1301 Protein_3295 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(10-109)

Antitoxin

(7-51)


Sequences


Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T101172 WP_000254745.1 NZ_CP028429:c3337977-3337642 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T101172 NZ_CP028429:c3337977-3337642 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT101172 WP_000581937.1 NZ_CP028429:c3338225-3337977 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT101172 NZ_CP028429:c3338225-3337977 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3


Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References