10117

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2142431..2142945 Replicon chromosome
Accession NC_002745
Organism Staphylococcus aureus subsp. aureus N315

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag SA_RS10870 Protein ID WP_000581792.1
Coordinates 2142431..2142640 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag SA_RS10875 Protein ID WP_000138932.1
Coordinates 2142652..2142945 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SA_RS10850 (SA1886) 2137939..2139297 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
SA_RS10855 (SA1887) 2139312..2140382 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
SA_RS10860 (SA1888) 2140700..2141902 + 1203 WP_001109939.1 rod shape-determining protein RodA -
SA_RS10865 (SAS068) 2142166..2142303 - 138 WP_000828354.1 hypothetical protein -
SA_RS10870 (SA1889) 2142431..2142640 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
SA_RS10875 (SA1890) 2142652..2142945 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
SA_RS10880 (SA1891) 2143111..2144595 + 1485 WP_000571549.1 cardiolipin synthase -
SA_RS10885 (SA1892) 2144623..2145270 + 648 WP_001187630.1 HD domain-containing protein -
SA_RS10895 (SA1893) 2146083..2146955 - 873 WP_000725802.1 membrane protein insertase YidC -
SA_RS10900 (SA1894) 2147041..2147682 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T10117 WP_000581792.1 NC_002745:c2142640-2142431 [Staphylococcus aureus subsp. aureus N315]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T10117 NC_002745:c2142640-2142431 [Staphylococcus aureus subsp. aureus N315]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT10117 WP_000138932.1 NC_002745:c2142945-2142652 [Staphylococcus aureus subsp. aureus N315]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT10117 NC_002745:c2142945-2142652 [Staphylococcus aureus subsp. aureus N315]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References

(1) Fuminori Kato et al. (2019) Genome-Wide Screening for Identification of Novel Toxin-Antitoxin Systems in Staphylococcus aureus. Applied And Environmental Microbiology 85(20):e00915-19. [PubMed:31375497] experimental


experimental experimental literature