Detailed information of TA system TA101144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1020473..1020971
• Replicon: chromosome
• Accession: NZ_CP028414
• Organism: Streptococcus mitis NCTC 12261

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F9HDX7
• Locus tag: SM12261_RS05280
• Protein ID: WP_004239453.1
• Coordinates: 1020473..1020727 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F9HDX8
• Locus tag: SM12261_RS05285
• Protein ID: WP_000208079.1
• Coordinates: 1020729..1020971 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM12261_RS05260	1015483..1017384	-	1902	WP_000584858.1	ABC-F family ATP-binding cassette domain-containing protein	-
SM12261_RS05265	1017538..1019256	+	1719	WP_000121686.1	phospho-sugar mutase	-
SM12261_RS05270	1019368..1019715	+	348	WP_000434630.1	thiol reductase thioredoxin	-
SM12261_RS05275	1019753..1020430	-	678	WP_000566125.1	ion transporter	-
SM12261_RS05280	1020473..1020727	-	255	WP_004239453.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SM12261_RS05285	1020729..1020971	-	243	WP_000208079.1	hypothetical protein	Antitoxin
SM12261_RS05290	1021062..1021871	-	810	WP_000004960.1	MBL fold metallo-hydrolase	-
SM12261_RS05295	1021864..1023222	-	1359	WP_000571163.1	cell wall metabolism sensor histidine kinase VicK	-
SM12261_RS05300	1023215..1023922	-	708	WP_000722072.1	response regulator transcription factor	-
SM12261_RS05305	1023978..1025153	-	1176	WP_000886130.1	A/G-specific adenine glycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-84)

Antitoxin

• Protein of unknown function DUF6290 (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10112.82 Da
• Isoelectric Point: 10.7632

>T101144 WP_004239453.1 NZ_CP028414:c1020727-1020473 [Streptococcus mitis NCTC 12261]
MYKLVPTRRFIKQLKKLDRYTQKIITNYLQTNVLEDPRRHGKALVGNRVGQWRYRIGNYRVIVQIVDDELVIATLEVGHR
RDIY

Nucleotide

• Length: 255 bp

>T101144 NZ_CP028414:c1020727-1020473 [Streptococcus mitis NCTC 12261]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGATCGTTATACGCAGAAGATAATTACAAA
CTATTTACAAACCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTCATTGCTACTTTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9206.40 Da
• Isoelectric Point: 3.9338

>AT101144 WP_000208079.1 NZ_CP028414:c1020971-1020729 [Streptococcus mitis NCTC 12261]
MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYDARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLEDE

Nucleotide

• Length: 243 bp

>AT101144 NZ_CP028414:c1020971-1020729 [Streptococcus mitis NCTC 12261]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGGGTTTCCCT
GTCGGAACTTATCCGCACCAAAACTCTTGAAGCTCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGGAGGATGAA
TAA

Structures

Toxin

• AlphaFold DB: F9HDX7

Antitoxin

• AlphaFold DB: F9HDX8