Detailed information of TA system TA101143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 662071..663317
• Replicon: chromosome
• Accession: NZ_CP028414
• Organism: Streptococcus mitis NCTC 12261

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: A0A380KWA4
• Locus tag: SM12261_RS03575
• Protein ID: WP_000405376.1
• Coordinates: 662547..663317 (+)
• Length: 257 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: SM12261_RS03570
• Protein ID: WP_000579609.1
• Coordinates: 662071..662547 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM12261_RS03545	657086..658834	+	1749	WP_001254284.1	ABC transporter ATP-binding protein/permease	-
SM12261_RS03550	659092..660351	+	1260	WP_000870440.1	TRZ/ATZ family protein	-
SM12261_RS03560	660602..661102	+	501	WP_001287267.1	50S ribosomal protein L10	-
SM12261_RS03565	661175..661543	+	369	WP_001196965.1	50S ribosomal protein L7/L12	-
SM12261_RS03570	662071..662547	+	477	WP_000579609.1	helix-turn-helix domain-containing protein	Antitoxin
SM12261_RS03575	662547..663317	+	771	WP_000405376.1	zeta toxin family protein	Toxin
SM12261_RS03585	664974..665405	+	432	WP_025169729.1	hypothetical protein	-
SM12261_RS03590	665408..665857	+	450	WP_000370556.1	hypothetical protein	-
SM12261_RS03595	665960..666430	+	471	WP_000447038.1	winged helix DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	638459..693605	55146

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 257 a.a.
• Molecular weight: 29394.27 Da
• Isoelectric Point: 7.2202

>T101143 WP_000405376.1 NZ_CP028414:662547-663317 [Streptococcus mitis NCTC 12261]
VEIQDYTDSEFKHALERNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVRELSHLGYNLLIEGTLRTIDVPKKTAKLLKNKGYEVQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQSEELAIFERIQIYQRDRSCVYDSGEDKTSAATVLHDLLFGEWNQVEKEML
KSGEERLKDLTNRNDC

Nucleotide

• Length: 771 bp

>T101143 NZ_CP028414:662547-663317 [Streptococcus mitis NCTC 12261]
GTGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGAGCGGAATCTTCGTTCTCTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTACTTGGAGGACAAAGTGGTGCTGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGCGATAGTTTTCGCTCTCAACATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATACACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAAGAGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACGATTGATGTTCCAAAGAAAACAGCAAAACTCTTGAAAAATA
AGGGGTATGAGGTACAATTAGCCTTAATTGCGACAAAGCCTAAGCTGTCCTATCTGAGTACTCTTATCCGATACGAAGAA
CTGTACGCTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGGCAATCGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTATCAACGAGATAGAAGTTGTGTATATGATTCAG
GAGAAGATAAAACTTCAGCAGCAACCGTCCTTCATGATTTACTATTTGGAGAATGGAATCAAGTTGAGAAAGAGATGCTT
AAATCTGGAGAAGAAAGATTGAAAGATTTAACGAATCGAAATGATTGTTAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18227.67 Da
• Isoelectric Point: 4.3457

>AT101143 WP_000579609.1 NZ_CP028414:662071-662547 [Streptococcus mitis NCTC 12261]
MIGKNIKSLRKTHDLTQPEFAQIIGISRNSLSRYENGTSPVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT101143 NZ_CP028414:662071-662547 [Streptococcus mitis NCTC 12261]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCCCAAATTATAGGAATTTC
TCGCAATAGCTTGAGTCGTTATGAAAATGGAACTAGTCCAGTCTCTACGGAACTAATAGATATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGAGCTAATCTATTATCACGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTTTCTGATTTGATTCATACGAATATCTATCTAGTTGAAACTTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAACGGATGGTAGCCTAG

Structures

Toxin

• AlphaFold DB: A0A380KWA4