Detailed information of TA system TA101141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5297359..5297990
• Replicon: chromosome
• Accession: NZ_CP028391
• Organism: Klebsiella pneumoniae strain WCHKP13F2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: B7D34_RS28730
• Protein ID: WP_002882817.1
• Coordinates: 5297359..5297742 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: B7D34_RS28735
• Protein ID: WP_002882814.1
• Coordinates: 5297742..5297990 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D34_RS28715	5294725..5295627	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
B7D34_RS28720	5295624..5296259	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
B7D34_RS28725	5296256..5297185	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
B7D34_RS28730	5297359..5297742	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7D34_RS28735	5297742..5297990	-	249	WP_002882814.1	ribbon-helix-helix domain-containing protein	Antitoxin
B7D34_RS28740	5298189..5299106	+	918	WP_004178029.1	alpha/beta hydrolase	-
B7D34_RS28745	5299120..5300109	-	990	WP_002882811.1	fatty acid biosynthesis protein FabY	-
B7D34_RS28750	5300106..5300543	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B7D34_RS28755	5300540..5301400	-	861	WP_004146232.1	virulence factor BrkB family protein	-
B7D34_RS28760	5301394..5301993	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (18-49)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T101141 WP_002882817.1 NZ_CP028391:c5297742-5297359 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T101141 NZ_CP028391:c5297742-5297359 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9786.10 Da
• Isoelectric Point: 4.3509

>AT101141 WP_002882814.1 NZ_CP028391:c5297990-5297742 [Klebsiella pneumoniae]
MSMMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRK
EW

Nucleotide

• Length: 249 bp

>AT101141 NZ_CP028391:c5297990-5297742 [Klebsiella pneumoniae]
ATGAGCATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCT
CAAAGTGCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTA
AAGATACCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAG
GAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8