Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | TsaAT/- |
| Location | 858892..859369 | Replicon | chromosome |
| Accession | NC_002745 | ||
| Organism | Staphylococcus aureus subsp. aureus N315 | ||
Toxin (Protein)
| Gene name | TsaT | Uniprot ID | T1Y7S2 |
| Locus tag | SA_RS04270 | Protein ID | WP_000052076.1 |
| Coordinates | 858892..859080 (-) | Length | 63 a.a. |
Antitoxin (Protein)
| Gene name | TsaA | Uniprot ID | T1Y6P8 |
| Locus tag | SA_RS04275 | Protein ID | WP_001059690.1 |
| Coordinates | 859109..859369 (-) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SA_RS04240 (SA0745) | 854886..855407 | + | 522 | WP_000824482.1 | hypothetical protein | - |
| SA_RS04245 (SA0746) | 855748..856434 | + | 687 | WP_000141557.1 | thermonuclease family protein | - |
| SA_RS04250 (SA0747) | 856791..856991 | + | 201 | WP_001059082.1 | cold-shock protein | - |
| SA_RS04255 (SA0748) | 857483..857701 | - | 219 | WP_000005886.1 | hypothetical protein | - |
| SA_RS04260 (SA0749) | 857763..858047 | - | 285 | WP_001004399.1 | hypothetical protein | - |
| SA_RS04265 (SA0750) | 858135..858704 | - | 570 | WP_001106654.1 | hypothetical protein | - |
| SA_RS04270 (SAS018) | 858892..859080 | - | 189 | WP_000052076.1 | hypothetical protein | Toxin |
| SA_RS04275 (SAS019) | 859109..859369 | - | 261 | WP_001059690.1 | hypothetical protein | Antitoxin |
| SA_RS04280 (SA0751) | 859710..859913 | + | 204 | WP_000145163.1 | sterile alpha motif-like domain-containing protein | - |
| SA_RS04285 (SA0752) | 859910..860146 | - | 237 | WP_000349581.1 | hypothetical protein | - |
| SA_RS04290 (SAS020) | 860371..860952 | + | 582 | WP_001140693.1 | phosphoglycerate mutase family protein | - |
| SA_RS04295 (SA0753) | 861025..861642 | - | 618 | WP_000255551.1 | LysE/ArgO family amino acid transporter | - |
| SA_RS04300 (SA0754) | 861797..862291 | - | 495 | WP_000569884.1 | GNAT family N-acetyltransferase | - |
| SA_RS04305 (SA0755) | 862690..863112 | - | 423 | WP_000974460.1 | organic hydroperoxide resistance protein | - |
| SA_RS04310 (SA0756) | 863260..863976 | + | 717 | WP_000150011.1 | type I 3-dehydroquinate dehydratase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 63 a.a. Molecular weight: 7142.82 Da Isoelectric Point: 9.9971
>T10114 WP_000052076.1 NC_002745:c859080-858892 [Staphylococcus aureus subsp. aureus N315]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL
Download Length: 189 bp
>T10114 NC_002745:c859080-858892 [Staphylococcus aureus subsp. aureus N315]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9940.09 Da Isoelectric Point: 4.0624
>AT10114 WP_001059690.1 NC_002745:c859369-859109 [Staphylococcus aureus subsp. aureus N315]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG
Download Length: 261 bp
>AT10114 NC_002745:c859369-859109 [Staphylococcus aureus subsp. aureus N315]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0VP18 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0VN56 |
References
(1) Fuminori Kato et al. (2019) Genome-Wide Screening for Identification of Novel Toxin-Antitoxin Systems in Staphylococcus aureus. Applied And Environmental Microbiology 85(20):e00915-19. [PubMed:31375497]