Detailed information of TA system TA101138

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4738375..4739078
• Replicon: chromosome
• Accession: NZ_CP028391
• Organism: Klebsiella pneumoniae strain WCHKP13F2

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: B7D34_RS25940
• Protein ID: WP_094617794.1
• Coordinates: 4738375..4738716 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: B7D34_RS25945
• Protein ID: WP_094617792.1
• Coordinates: 4738737..4739078 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D34_RS25905	4733586..4734260	+	675	WP_004152211.1	hypothetical protein	-
B7D34_RS25910	4734262..4734609	+	348	WP_004199257.1	hypothetical protein	-
B7D34_RS25915	4734612..4735091	+	480	WP_002887049.1	type VI secretion system tube protein Hcp	-
B7D34_RS25920	4735283..4735606	-	324	Protein_4629	superfamily I DNA helicase	-
B7D34_RS25925	4735625..4736736	-	1112	Protein_4630	IS3 family transposase	-
B7D34_RS25930	4736823..4737027	-	205	Protein_4631	hypothetical protein	-
B7D34_RS25935	4737104..4738111	-	1008	WP_049017154.1	restriction endonuclease	-
B7D34_RS25940	4738375..4738716	-	342	WP_094617794.1	TA system toxin CbtA family protein	Toxin
B7D34_RS25945	4738737..4739078	-	342	WP_094617792.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B7D34_RS25950	4739089..4739631	-	543	WP_087696572.1	DNA repair protein RadC	-
B7D34_RS25955	4739644..4740084	-	441	WP_094617790.1	antirestriction protein	-
B7D34_RS25960	4740115..4740936	-	822	WP_094617788.1	DUF945 domain-containing protein	-
B7D34_RS25965	4741056..4741529	-	474	WP_094617786.1	hypothetical protein	-
B7D34_RS25970	4741601..4742053	-	453	WP_087696570.1	hypothetical protein	-
B7D34_RS25975	4742089..4742805	-	717	WP_087696569.1	WYL domain-containing protein	-
B7D34_RS25980	4743049..4743924	-	876	WP_094617784.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4733586..4762474	28888

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12753.65 Da
• Isoelectric Point: 8.0324

>T101138 WP_094617794.1 NZ_CP028391:c4738716-4738375 [Klebsiella pneumoniae]
MKTLPATNPQAATLCLSSVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQTTGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T101138 NZ_CP028391:c4738716-4738375 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAAATCCGCAGGCGGCGACGCTATGTCTGTCATCCGTGGCAGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCGTTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGACAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12676.38 Da
• Isoelectric Point: 6.7300

>AT101138 WP_094617792.1 NZ_CP028391:c4739078-4738737 [Klebsiella pneumoniae]
MKSTDSESDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFNEAECLKLDAAFPHLICQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTHR

Nucleotide

• Length: 342 bp

>AT101138 NZ_CP028391:c4739078-4738737 [Klebsiella pneumoniae]
ATGAAATCTACTGATTCAGAGAGCGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTGCACTATTTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAACGAAGCAGAATGTC
TTAAGCTGGATGCGGCATTCCCGCATTTAATCTGCCAGATGGAATCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACCCCACTCATCGTTAA